M-CSF

Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute in sterile distilled water to 0.1-0.5 mg/ml.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- The ED50 was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.
- Purity
- >98% by SDS PAGE and HPLC analyses
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P09603
- Entrez Gene
- CSF1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001954 positive regulation of cell-matrix adhesion
- GO:0003006 developmental process involved in reproduction
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005157 macrophage colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0008083 growth factor activity
- GO:0008283 cell proliferation
- GO:0010628 positive regulation of gene expression
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0030225 macrophage differentiation
- GO:0030316 osteoclast differentiation
- GO:0030335 positive regulation of cell migration
- GO:0032270 positive regulation of cellular protein metabolic process
- GO:0032946 positive regulation of mononuclear cell proliferation
- GO:0042117 monocyte activation
- GO:0042803 protein homodimerization activity
- GO:0043235 receptor complex
- GO:0045651 positive regulation of macrophage differentiation
- GO:0045657 positive regulation of monocyte differentiation
- GO:0045860 positive regulation of protein kinase activity
- GO:0048471 perinuclear region of cytoplasm
- Regulatory
- For research purposes only
Applications of M-CSF
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Functional Assays |
Product Specific References
References for M-CSF
-
Ralph, P. et al. (1986) Molecular and biological properties of human macrophage growth factor, CSF-1.
Cold Spring Harb Symp Quant Biol. 51:679 - 683 -
MacDonald, B.R. et al. (1986) Effects of human recombinant CSF-GM and highly purified CSF-1 on the formation of multinucleated cells with osteoclast characteristics in long-term bone marrow cultures
J Bone Miner Res. 1: 227 - 233. -
Ralph, P. et al. (1986) Biological properties and molecular biology of the human macrophage growth
factor, CSF-1.
Immunobiology. 172: 194 - 204. -
Wong, G.G. (1987) Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human
urinary protein.
Science. 235: 1504-1508
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra