Recombinant human macrophage colony stimulating factor (M-CSF) expressed in E. coli.
- Target Species
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitute in sterile distilled water to 0.1-0.5 mg/ml.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- The ED50 was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.
- >98% by SDS PAGE and HPLC analyses
- Endotoxin Level
- < 0.1 ng/ug
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
- Entrez Gene
- GO Terms
positive regulation of cell-matrix adhesion
developmental process involved in reproduction
integral to membrane
macrophage colony-stimulating factor receptor binding
growth factor activity
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of cell migration
positive regulation of cellular protein metabolic process
positive regulation of mononuclear cell proliferation
protein homodimerization activity
positive regulation of macrophage differentiation
positive regulation of monocyte differentiation
positive regulation of protein kinase activity
perinuclear region of cytoplasm
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for M-CSF
Ralph, P. et al. (1986) Molecular and biological properties of human macrophage growth factor, CSF-1.
Cold Spring Harb Symp Quant Biol. 51:679 - 683
MacDonald, B.R. et al. (1986) Effects of human recombinant CSF-GM and highly purified CSF-1 on the formation of multinucleated cells with osteoclast characteristics in long-term bone marrow cultures
J Bone Miner Res. 1: 227 - 233.
Ralph, P. et al. (1986) Biological properties and molecular biology of the human macrophage growth
Immunobiology. 172: 194 - 204.
Wong, G.G. (1987) Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human
Science. 235: 1504-1508