M-CSF

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Recombinant Human M-CSF

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
4740-1255 Datasheet	E FN SDS	2 µg	Log in		Get Quote

Recombinant human macrophage colony stimulating factor (M-CSF) expressed in E. coli.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute in sterile distilled water to 0.1-0.5 mg/ml.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: The ED₅₀ was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is 1.0 ng/ml, corresponding to a specific activity of 1 x 10⁶ units/mg.
Purity: >98% by SDS PAGE and HPLC analyses
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P09603
Entrez Gene: CSF1
GO Terms: GO:0005886 plasma membrane; GO:0001954 positive regulation of cell-matrix adhesion; GO:0003006 developmental process involved in reproduction; GO:0016021 integral to membrane; GO:0005125 cytokine activity; GO:0005157 macrophage colony-stimulating factor receptor binding; GO:0005615 extracellular space; GO:0008083 growth factor activity; GO:0008283 cell proliferation; GO:0010628 positive regulation of gene expression; GO:0010744 positive regulation of macrophage derived foam cell differentiation; GO:0030225 macrophage differentiation; GO:0030316 osteoclast differentiation; GO:0030335 positive regulation of cell migration; GO:0032270 positive regulation of cellular protein metabolic process; GO:0032946 positive regulation of mononuclear cell proliferation; GO:0042117 monocyte activation; GO:0042803 protein homodimerization activity; GO:0043235 receptor complex; GO:0045651 positive regulation of macrophage differentiation; GO:0045657 positive regulation of monocyte differentiation; GO:0045860 positive regulation of protein kinase activity; GO:0048471 perinuclear region of cytoplasm
Regulatory: For research purposes only

Applications of M-CSF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for M-CSF

Ralph, P. et al. (1986) Molecular and biological properties of human macrophage growth factor, CSF-1.
Cold Spring Harb Symp Quant Biol. 51:679 - 683
MacDonald, B.R. et al. (1986) Effects of human recombinant CSF-GM and highly purified CSF-1 on the formation of multinucleated cells with osteoclast characteristics in long-term bone marrow cultures
J Bone Miner Res. 1: 227 - 233.
Ralph, P. et al. (1986) Biological properties and molecular biology of the human macrophage growth factor, CSF-1.
Immunobiology. 172: 194 - 204.
Wong, G.G. (1987) Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein.
Science. 235: 1504-1508

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