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M-CSF

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Recombinant Human M-CSF

Product Type
Recombinant Protein
Specificity
M-CSF

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4740-1255
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Recombinant human macrophage colony stimulating factor (M-CSF) expressed in E. coli.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute in sterile distilled water to 0.1-0.5 mg/ml.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
The ED50 was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.
Purity
>98% by SDS PAGE and HPLC analyses
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for M-CSF

  1. Ralph, P. et al. (1986) Molecular and biological properties of human macrophage growth factor, CSF-1.
    Cold Spring Harb Symp Quant Biol. 51:679 - 683
  2. MacDonald, B.R. et al. (1986) Effects of human recombinant CSF-GM and highly purified CSF-1 on the formation of multinucleated cells with osteoclast characteristics in long-term bone marrow cultures
    J Bone Miner Res. 1: 227 - 233.
  3. Ralph, P. et al. (1986) Biological properties and molecular biology of the human macrophage growth factor, CSF-1.
    Immunobiology. 172: 194 - 204.
  4. Wong, G.G. (1987) Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein.
    Science. 235: 1504-1508

UniProt
P09603
Entrez Gene
CSF1
GO Terms
GO:0005886 plasma membrane
GO:0001954 positive regulation of cell-matrix adhesion
GO:0003006 developmental process involved in reproduction
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005157 macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0010628 positive regulation of gene expression
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0030225 macrophage differentiation
GO:0030316 osteoclast differentiation
GO:0030335 positive regulation of cell migration
GO:0032270 positive regulation of cellular protein metabolic process
GO:0032946 positive regulation of mononuclear cell proliferation
GO:0042117 monocyte activation
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
GO:0045651 positive regulation of macrophage differentiation
GO:0045657 positive regulation of monocyte differentiation
GO:0045860 positive regulation of protein kinase activity
GO:0048471 perinuclear region of cytoplasm
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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