Low Density Lipoprotein

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Native Human Low Density Lipoprotein (Oxidized)

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5685-3557 FN 2 mg
Native oxidized Human Low Density Lipoprotein is provided, purified from human plasma in liquid form. (LDL) oxidative modification is one of the major processes involved in atherosclerosis along with other degenerative disorders. Oxidized LDL is preferentially accumulated by macrophages which become so called "foam cells" within the atheromatous plaque and contributes to the accumulation of cholesterol, typical of atherosclerosis.

Product Details

Target Species
Human
Product Form
Purified oxidised LDL from human plasma - liquid
Preparation
LDL is purified to homogeneity by ultra-centrifugation. Oxidised using 20 µM cupric sulphate in PBS at 37ºC for 24 hours. Oxidation is terminated with excess EDTA. The level of oxidation is measured using TBARS (Thiobarbituric acid reactive substances) determination using a Malondialdehyde (MDA) standard. The level of oxidation is batch specific and available on request.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.3 mM EDTA
Approx. Protein Concentrations
Total protein concentration 2.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Shelf Life
This product is stable for approximately 4 weeks when handled aseptically and stored at 4ºC. Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Low Density Lipoprotein

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Low Density Lipoprotein

  1. Yang, K. et al. (2015) Oxidized low-density lipoprotein promotes macrophage lipid accumulation via the toll-like receptor 4-Src pathway.
    Circ J. 79 (11): 2509-16.
  2. Wang, X. Q. et al. (2016) C1q/TNF-related protein 1 links macrophage lipid metabolism to inflammation and atherosclerosis
    Atherosclerosis. Apr 28 [Epub ahead of print]
  3. Dai, D. et al. (2016) Association of decreased serum sTREM-1 level with the severity of coronary artery disease: Inhibitory effect of sTREM-1 on TNF-α- and oxLDL-induced inflammatory reactions in endothelial cells.
    Medicine (Baltimore). 95 (37): e4693.
  4. Zhang, B-C. et al. (2016) Luteolin Attenuates Foam Cell Formation and Apoptosis in Ox-LDL-Stimulated Macrophages by Enhancing Autophagy.
    Cell Physiol Biochem. 39 (5): 2065-2076.
  5. Fukushi, S. et al. (2018) Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA.
    J Virol Methods. 251: 22-29.
  6. Carbone, M.L. et al. (2017) Leukocyte RhoA exchange factor Arhgef1 mediates vascular inflammation and atherosclerosis.
    J Clin Invest. Nov 13 [Epub ahead of print].