Low Density Lipoprotein

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Native Human Low Density Lipoprotein:Dii

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2550-0104 F IA IF 0.2 mg
DiI labelled human low densitiy lipoprotein has been used for monitoring Ac-LDL uptake by monocytes/ macrophages in conjunction with excess unlabelled Ac-LDL 5685-3404 (Rahman et al. 2008).

Sample lots of DiI labeled human low density lipoprotein are individually evaluated for the labeling of human skin fibroblasts or P-388D cells grown in lipoprotein deficient medium for 48 hours.

Product Details

Target Species
Human
Product Form
Purified from human serum - liquid
Preparation
Purified LDL is labelled with the fluorescent probe Dil (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate). The resultant product is then dialysed against 0.15 M NaCl, 0.05 M TRIS (pH 7.4) and 0.3 mM EDTA, sterilised by filtration and aseptically packaged.
Preservative Stabilisers
None present.
Approx. Protein Concentrations
Total protein concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Low Density Lipoprotein

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
In vitro Assay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Low Density Lipoprotein

  1. Barak, L.S. & Webb, W.W. (1981) Fluorescent low density lipoprotein for observation of dynamics of individual receptor complexes on cultured human fibroblasts.
    J. Cell Biol. 90: 595-604.
  2. Pitas, R.E. et al. (1983) Foam cells in explants of atherosclerotic rabbit aortas have receptors for beta-very low density lipoproteins and modified low density lipoproteins.
    Arteriosclerosis. 3: 2-12.
  3. Barak, L.S. & Webb, W.W. (1982) Diffusion of low density lipoprotein-receptor complex on human fibroblasts.
    J. Cell Biol. 95: 846-52.
  4. Rahman, E.U. et al. (2008) Mesangial matrix-activated monocytes express functional scavenger receptors and accumulate intracellular lipid.
    Nephrol Dial Transplant. 23: 1876-1885.
  5. Lufino, M.M. et al. (2016) The infectious BAC genomic DNA expression library: a high capacity vector system for functional genomics.
    Sci Rep. 6: 28644.