Low Density Lipoprotein

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Native Human Low Density Lipoprotein (Acetylated):Dil

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2550-0004 F FN IF* 0.2 mg
Many lots have been evaluated for the specific labelling of bovine aortic endothelium, HUVECS, murine macrophages and the current lot was evaluated on P-388D cells.

Product Details

Target Species
Human
Product Form
Purified acetylated LDL from human serum, Dil labelled - liquid
Preparation
Purified LDL is acetylated and then labelled with the fluorescent probe Dil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindo-carbocyanine perchlorate). The resultant product is exhaustively dialyzed against 0.15 M NaCl, 0.05 M Tris, pH 7.4, 0.3 mM EDTA, sterilized by filtration and then aseptically packaged.
Preservative Stabilisers
None present.
Approx. Protein Concentrations
Total protein concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Shelf Life
This product is stable when handled aseptically and stored at 4ÂșC. Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Low Density Lipoprotein

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Immunofluorescence 1
  1. 13% formaldehyde in PBS is required for fixation. Methanol or Acetone can not be used as 2550-0004 is soluble in organic solvents.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for Low Density Lipoprotein

  1. Fogelman, A. M. et al. (1980) Malondialdehyde alteration of low density lipoproteins leads to cholesteryl ester accumulation in human monocyte-macrophages.
    Proc. Natl. Acad. Sci. USA. 77: 2214-2218.
  2. Pitas, R. E. et al. (1981) Acetoacetylated lipoproteins used to distinguish fibroblasts from macrophages in vitro by fluorescence microscopy.
    Arteriosclerosis.1: 177-185.
  3. Voyta, J. C. et al. (1984) Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.
    J. Cell Biol.99: 2034-2040.
  4. Allen, S. et al. (1998) Native low density lipoprotein-induced calcium transients trigger VCAM-1 and E-selectin expression in cultured human vascular endothelial cells.
    J. Clin. Invest. 101: 351 - 361
  5. Gough, P.J., et al. (2001) The use of human CD68 transcriptional regulatory sequences to direct high-level expression of class A scavenger receptor in macrophages in vitro and in vivo.
    Immunology.103: 351-361.
  6. Stein, O & Stein, Y. (1980) Bovine aortic endothelial cells display macrophage-like properties towards acetylated 125I-labelled low density lipoprotein.
    Biochim Biophys Acta. 620: 631-635.
  7. Chiang, C.H. et al. (2011) Investigation of hepatoprotective activity of induced pluripotent stem cells in the mouse model of liver injury.
    J Biomed Biotechnol. 2011:219060.
  8. Garside, S.A. et al. (2010) Thrombospondin-1 inhibits angiogenesis and promotes follicular atresia in a novel in vitro angiogenesis assay.
    Endocrinology. 151: 1280-9.
  9. Rae, M. et al. (2009) Cortisol inactivation by 11beta-hydroxysteroid dehydrogenase-2 may enhance endometrial angiogenesis via reduced thrombospondin-1 in heavy menstruation.
    J Clin Endocrinol Metab. 94: 1443-50.