KGF

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Recombinant Human KGF

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP089 FN 10 µg
Recombinant Human Keratinocyte growth factor (KGF) is a potent mitogen for keratinocytes and epithelial cells.

Activity is assessed by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells.

Product Details

Target Species
Human
Product Form
Purified recombinant protein expressed in E. coli - lyophilised
Reconstitution
Reconstitute with 0.1 ml phosphate buffered saline pH7.0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Activity
1 x 105 units/mg
Purity
>96% by SDS PAGE and N-Terminal sequence analysis
Approx. Protein Concentrations
0.1 mg/ml
Protein Molecular Weight
19kDa (163 Amino acid sequence)

Storage Information

Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of reconstitution.

More Information

UniProt
P21781 Related reagents
Entrez Gene
FGF7 Related reagents
GO Terms
GO:0008083 growth factor activity
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010463 mesenchymal cell proliferation
GO:0010838 positive regulation of keratinocyte proliferation
GO:0031532 actin cytoskeleton reorganization
GO:0034394 protein localization at cell surface
GO:0042056 chemoattractant activity
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0051549 positive regulation of keratinocyte migration
GO:0051781 positive regulation of cell division
GO:0060501 positive regulation of epithelial cell proliferation involved in lung morphogenesis
GO:0061033 secretion by lung epithelial cell involved in lung growth
Regulatory
For research purposes only

Applications of KGF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 10ng/ml 100ng/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.