Inhibin Beta B

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Recombinant Human Inhibin Beta B

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP240 FN 5 µg
Inhibin Beta B, otherwise known as Activin B, is a member of the transforming growth factor-beta (TGF-B) family, which signals through the ActRII receptor (Activin Receptor type II), and acts as a regulator of hormone secretion from the pituitary, hypothalamic and gonadal glands.

Inhibin Beta B is a homodimer of the inhibin/activin beta-B chain, and is involved in several aspects of embryonic development, as well as haematopoiesis and osteogenesis, and also acts as a modulator of FSH (follicle-stimulating hormone) secretion and haemoglobin synthesis.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 10ul 10mM sodium citrate pH 3,0.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant Inhibin Beta B expressed in High-5 insect cells
Buffer Solution
10mM Sodium citrate.
Preservative Stabilisers
5% Trehalose
Activity
The ED50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is ≤ 2ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Purity
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.5 mg/ml after reconstitution
Molecular Weight
25.6kD (115 amino acid Beta B homodimer)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P09529 Related reagents
Entrez Gene
INHBB Related reagents
GO Terms
GO:0001541 ovarian follicle development
GO:0006952 defense response
GO:0005576 extracellular region
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0040007 growth
GO:0008083 growth factor activity
GO:0009267 cellular response to starvation
GO:0046881 positive regulation of follicle-stimulating hormone secretion
GO:0046882 negative regulation of follicle-stimulating hormone secretion
GO:0032869 cellular response to insulin stimulus
GO:0032924 activin receptor signaling pathway
GO:0046789 host cell surface receptor binding
GO:0042803 protein homodimerization activity
GO:0045444 fat cell differentiation
GO:0046676 negative regulation of insulin secretion
GO:0048178 negative regulation of hepatocyte growth factor biosynthetic process
GO:0048471 perinuclear region of cytoplasm
GO:0060279 positive regulation of ovulation
Regulatory
For research purposes only

Applications of Inhibin Beta B

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.