IL-8

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Recombinant Human Interleukin-8 (72 Amino Acids)

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP055 E FN WB 25 µg
Recombinant Human interleukin-8 (72aa) is an E. coli derived form of human interleukin-8, also known as Interleikin-8 (6-77), (Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF-c or Neutrophil-activating factor. The product is a truncated form of full length recombinant human Interleukin-8 (PHP054 ) lacking the five amino terminal residues (AVLPR). Significant chemotactic activity of Human IL-8 on human neutrophils was achieved at 25ng/ml (assayed by a modified Boyden chamber).

Product Details

Target Species
Human
Product Form
Purified protein produced in E. coli - lyophilised
Reconstitution
Reconstitute with 0.25ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preservative Stabilisers
None present.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution.
Protein Molecular Weight
8.4kD (72 Amino acid sequence)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P10145 Related reagents
Entrez Gene
IL8 Related reagents
Regulatory
For research purposes only

Applications of IL-8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 10 100ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
ELISA
Recombinant human IL-8 may be used as the standard for ELISA applications with either a purified human IL-8 antibody (AHP781) or a biotinylated human IL-8 antibody (AHP781B)
Western Blotting
Recombinant human IL-8 may be used as the positive control for Western Blotting applications with either a purified human IL-8 antibody (AHP781) or a biotinylated human IL-8 antibody (AHP781B).