IL-8

Recombinant Human Interleukin-8 (72 Amino Acids)

Product Type
Recombinant Protein
Specificity
IL-8
Region
(72 AMINO ACIDS)

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PHP055
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Recombinant Human interleukin-8 (72aa) is an E. coli derived form of human interleukin-8, also known as Interleikin-8 (6-77), (Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF-c or Neutrophil-activating factor. Recombinant Human interleukin-8 (72aa) is a truncated form of full length recombinant human Interleukin-8 lacking the five amino terminal residues (AVLPR). Significant chemotactic activity of Human IL-8 on human neutrophils was achieved at 25ng/ml (assayed by a modified Boyden chamber).

Target Species
Human
Product Form
Purified protein produced in E. coli - lyophilized
Reconstitution
Reconstitute with 0.25ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preservative Stabilisers
None present.
Carrier Free
Yes
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution.
Protein Molecular Weight
8.4kD (72 Amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 10 100ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
ELISA
Recombinant human IL-8 may be used as the standard for ELISA applications with either a purified human IL-8 antibody (AHP781) or a biotinylated human IL-8 antibody (AHP781B)
Western Blotting
Recombinant human IL-8 may be used as the positive control for Western Blotting applications with either a purified human IL-8 antibody (AHP781) or a biotinylated human IL-8 antibody (AHP781B).

Synonyms
CXCL8
UniProt
P10145
Entrez Gene
IL8
View more products with IL-8 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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