IL-32A

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Recombinant Human INTERLEUKIN-32A

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP301
Datasheet
E
SDS
10 µg loader

Recombinant Human interluekin-32A

Interleukin-32 alpha (IL-32A), also known as NK4 and TAIF is a pro-inflammatory cytokine with roles in inflammation and immune regulation, and its expression is strongly regulated by pathogens and pro-inflammatory cytokines (Hong et al. 2017). IL-32 A promotes inflammation by upregulating other inflammatory cytokines including TNF-α, IL-1β, IL-6, INF-γ, and IL-8 (Al-Shobaili et al. 2018). Accordingly, a role for IL-32A has been reported in the pathogenesis of chronic inflammatory diseases including IBD, COPD and RA (Damen et al. 2017). IL-32A is highly expressed in several cancer tissues, and plays a crucial role in cancer development through tumor initiation, cell proliferation, and tumor maintenance (Yan et al. 2018).

This recombinant Human interleukin-32 alpha isoform can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-32 antibody, clone E03-4C9 (MCA6212GA) as a capture antibody and biotinylated Mouse anti Human interleukin-32 antibody, clone B01-2A7 (MCA6213B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Recombinant protein expressed in E. coli and purified by ion exchange chromatography
Buffer Solution
50 mM Tris-HCl, pH8.0, 0.15 M NaCl
Preservative Stabilisers
1.0% Trehalose
Purity
≥90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
Approx. Protein Concentrations
100 μg/ml after reconstitution
Protein Molecular Weight
14.9 kDa

Storage Information

Storage
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
P24001
Entrez Gene
IL32
GO Terms
GO:0006952 defense response
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IL-32A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Interleukin-32 MCA6212GA E 0.1 mg loader
Mouse anti Human Interleukin-32:Biotin MCA6213B E 0.1 mg loader

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