IL-3

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Recombinant Human Interleukin-3

Product Type: Recombinant Protein
Specificity: IL-3

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References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

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PHP043 Datasheet Datasheet SDS SDS	FN	10 µg		Log in	Get Quote
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Human interleukin-3 is an E. coli-derived recombinant protein corresponding to full-length mature human interleukin-3. Interleukin-3 supports the formation of multilineage colonies in the early development of multipotent haematopoietic progenitor cells. It has been shown to induce colony formation of macrophages, neutrophils, mast cells and megakaryocytes from agar-suspended bone marrow cells.

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Preparation: Recombinant protein expressed in E. coli.
Source: E.coli
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: >1 x 10⁷ units/mg
Purity: >98% by SDS PAGE analysis and N terminal sequence analysis
Protein Molecular Weight: 15kDa (133 amino acids)
Endotoxin Level: < 0.1 ng/ug
Regulatory: For research purposes only
Guarantee: 3 months from date of reconstitution

Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays		0.1ng/ml	1.0ng/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IL-3

Matsuyama, N. et al. (2015) The IgE-dependent pathway in allergic transfusion reactions: involvement of donor blood allergens other than plasma proteins.
Int J Hematol. 102 (1): 93-100.

UniProt: P08700
Entrez Gene: IL3
GO Terms: GO:0005125 cytokine activity; GO:0005135 interleukin-3 receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0007267 cell-cell signaling; GO:0007399 nervous system development; GO:0008083 growth factor activity; GO:0008284 positive regulation of cell proliferation; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; View More GO Terms; GO:0045740 positive regulation of DNA replication; GO:0045885 positive regulation of survival gene product expression