IL-3

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Recombinant Human Interleukin-3

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP043 FN 10 µg
Human interleukin-3 is an E. coli-derived recombinant protein corresponding to full-length mature human interleukin-3. Interleukin-3 supports the formation of multilineage colonies in the early development of multipotent haematopoietic progenitor cells. It has been shown to induce colony formation of macrophages, neutrophils, mast cells and megakaryocytes from agar-suspended bone marrow cells.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Preparation
Recombinant protein expressed in E. coli.
Preservative Stabilisers
None present
Activity
>1 x 107 units/mg
Purity
>98% by SDS PAGE analysis and N terminal sequence analysis
Protein Molecular Weight
15kDa (133 amino acids)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P08700 Related reagents
Entrez Gene
IL3 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005135 interleukin-3 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0007399 nervous system development
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045740 positive regulation of DNA replication
GO:0045885 positive regulation of survival gene product expression
Regulatory
For research purposes only

Applications of IL-3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 0.1ng/ml 1.0ng/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IL-3

  1. Matsuyama, N. et al. (2015) The IgE-dependent pathway in allergic transfusion reactions: involvement of donor blood allergens other than plasma proteins.
    Int J Hematol. 102 (1): 93-100.