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IL-17A

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Recombinant Human Interleukin-17A

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP294
Datasheet
E
MSDS
0.2 mg loader

Recombinant Human Interleukin-17A (IL-17A) is a proinflammatory cytokine produced by activated T lymphocytes. It forms a disulfide-linked homodimer; the monomer subunit is ~15 kDa. Elevated levels of IL-17A are linked with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. This product has been demonstrated for use in a pharmacokinetic antigen capture ELISA with the monoclonal antibody drug secukinumab, using an anti-secukinumab antibody for detection.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 0.5 mg/ml by adding 0.4 ml ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Mix gently after reconstitution. Do not vortex.
Preparation
Recombinant protein expressed in E. coli and purified by affinity chromatography
Buffer Solution
20 mM Citrate Buffer, pH4.5, 0.1 M Sodium Chloride
Preservative Stabilisers
1% Trehalose
Purity
≥90% determined by SDS-PAGE under reducing conditions and visualized by coomassie blue staining
Approx. Protein Concentrations
0.5 mg/ml after reconstitution
Protein Molecular Weight
Predicted 15 kDa. The apparent molecular mass of the human IL-17A is approximately 15 kDa determined by SDS-PAGE under reducing conditions.

Storage Information

Storage
Prior to reconstitution store at -70oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
Q16552
Entrez Gene
IL17A
GO Terms
GO:0001775 cell activation
GO:0006915 apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006486 protein glycosylation
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0032747 positive regulation of interleukin-23 production
GO:0045672 positive regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Regulatory
For research purposes only

Applications of IL-17A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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