IL-17A
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, the hIL-17A/secukinumab complex was formed by adding 5 µg/ml secukinumab to the wells coated with hIL-17A (PHP294). Detection was performed using Human Anti-Secukinumab (Drug/Target Complex) Antibody, clone AbD36949 (HCA375) at a concentration of 2 μg/ml followed by an HRP conjugated anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with secukinumab, hIL-17A (PHP294), human IgG1 kappa, and human IgG1 lambda at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, the hIL-17A/secukinumab complex was formed by adding 2 µg/ml secukinumab to the wells coated with hIL-17A (PHP294). Detection was performed using Human Anti-Secukinumab (Drug/Target Complex) Antibody, clone AbD36949 (HCA375) titrated to the given concentrations in PBST followed by Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with human IL-17A (PHP294) at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of secukinumab. Detection was performed using Human Anti-Secukinumab (Drug/Target Complex) Antibody, clone AbD36949 (HCA375) at a concentration of 2 µg/ml followed by Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
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E ResetRecombinant Human Interleukin-17A
- Product Type
- Recombinant Protein
- Specificity
- IL-17A
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| Recombinant Human Interleukin-17A (IL-17A) is a proinflammatory cytokine produced by activated T lymphocytes. It forms a disulfide-linked homodimer; the monomer subunit is ~15 kDa. Elevated levels of IL-17A are linked with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. This product has been demonstrated for use in a pharmacokinetic antigen capture ELISA with the monoclonal antibody drug secukinumab, using an anti-secukinumab antibody for detection. |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Centrifuge vial prior to reconstitution. Reconstitute to 0.5 mg/ml by adding 0.4 ml ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Mix gently after reconstitution. Do not vortex. - Preparation
- Recombinant protein expressed in E. coli and purified by affinity chromatography
- Buffer Solution
- 20 mM Citrate Buffer, pH4.5, 0.1 M Sodium Chloride
- Preservative Stabilisers
- 1% Trehalose
- Purity
- ≥90% determined by SDS-PAGE under reducing conditions and visualized by coomassie blue staining
- Approx. Protein Concentrations
- 0.5 mg/ml after reconstitution
- Protein Molecular Weight
- Predicted 15 kDa. The apparent molecular mass of the human IL-17A is approximately 15 kDa determined by SDS-PAGE under reducing conditions.
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -70oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- Q16552
- Entrez Gene
- IL17A
- GO Terms
- GO:0001775 cell activation
- GO:0006915 apoptosis
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006486 protein glycosylation
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0032747 positive regulation of interleukin-23 production
- View More GO Terms
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
View more products with IL-17 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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