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IL-17

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Recombinant Human Interleukin-17

Product Type
Recombinant Protein
Specificity
IL-17

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
PHP098
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 25 µg loader
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loader
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Recombinant Human interleukin-17is a preparation of human interleukin-17 prepared in E. coli. Interleukin-17 is a disulphide-linked homodimer of two ~15.5 kDa subunits. High levels of IL-17 homodimer are produced by stimulated primary CD4 the T-cells from the peripheral blood. IL-17 is reported to enhance the expression of intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts.

Target Species
Human
Product Form
Purified recombinant protein expressed in E. coli - lyophilized
Reconstitution
Reconstitute with 25 ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
3 months from date of reconstitution

Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 1ng/ml 2ng/ml
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP098 may be used as a standard in an ELISA with AHP455G.
Western Blotting
In Western blotting PHP098 represents as a suitable positive control for use with AHP455G.

Synonyms
IL-17A
UniProt
Q16552
Entrez Gene
IL17A
GO Terms
GO:0001775 cell activation
GO:0006915 apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006486 protein glycosylation
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0032747 positive regulation of interleukin-23 production
GO:0045672 positive regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
View more products with IL-17 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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