IL-17

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Recombinant Human Interleukin-17

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PHP098	E FN WB			25 µg

Summary

Recombinant Human interleukin-17is a preparation of human interleukin-17 prepared in E. coli. Interleukin-17 is a disulphide-linked homodimer of two ~15.5 kDa subunits. High levels of IL-17 homodimer are produced by stimulated primary CD4 the T-cells from the peripheral blood. IL-17 is reported to enhance the expression of intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts.

Product Details

Target Species: Human
Product Form: Purified recombinant protein expressed in E. coli - lyophilised
Reconstitution: Reconstitute with 25 ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers: None present
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 1.0 mg/ml after reconstitution
Endotoxin Level: <0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

More Information

UniProt: Q16552 Related reagents
Entrez Gene: IL17A Related reagents
GO Terms: GO:0001775 cell activation; GO:0006915 apoptosis; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0006486 protein glycosylation; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0007267 cell-cell signaling; GO:0032747 positive regulation of interleukin-23 production; GO:0045672 positive regulation of osteoclast differentiation; GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Regulatory: For research purposes only

Applications of IL-17

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Functional Assays	1ng/ml	2ng/ml
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PHP098 may be used as a standard in a sandwich ELISA with AHP455G as the capture antibody and AHP455B as the detection antibody.
Western Blotting: In Western blotting PHP098 represents as suitable positive control for use with AHP455G or AHP455B.

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