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IL-15

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Recombinant Human Interleukin-15

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP099A
Datasheet
FN
MSDS
10 µg loader

This product is an E. coli-derived recombinant protein corresponding to full-length mature human interleukin-15 (IL-15), a cytokine that stimulates proliferation of T-lymphocytes.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 ul distilled water. For extended storage the addition of 0.1% BSA and 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For functional studies do not add sodium azide
Buffer Solution
Sodium Phosphate
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
PHP099A has a specific activity of > 2 x 106 units/mg as determined by dose-dependent proliferation assay of CTLL-2 cells.
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml after reconstitution
Protein Molecular Weight
12.9 kDa based on 115 amino acid sequence
Endotoxin Level
< 0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
3 months from date of reconstitution

More Information

UniProt
P40933
Entrez Gene
IL15
GO Terms
GO:0004871 signal transducer activity
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005634 nucleus
GO:0005768 endosome
GO:0005794 Golgi apparatus
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0032740 positive regulation of interleukin-17 production
GO:0034105 positive regulation of tissue remodeling
GO:0050729 positive regulation of inflammatory response
Regulatory
For research purposes only

Applications of IL-15

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 0.1ng/ml 10ng/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
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