IL-15

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Recombinant Human Interleukin-15

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PHP099A	FN			10 µg

Summary

This product is an E. coli-derived recombinant protein corresponding to full-length mature human Interleukin-15 (IL-15), a cytokine that stimulates proliferation of T-lymphocytes

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilised.
Reconstitution: Reconstitute with 20 ul distilled water. For extended storage the addition of 0.1% BSA and 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For functional studies do not add sodium azide
Buffer Solution: Sodium Phosphate
Preservative Stabilisers: None present
Activity: PHP099A has a specific activity of > 2 x 10⁶ units/mg as determined by dose-dependent proliferation assay of CTLL-2 cells.
Purity: >98% by SDS PAGE and HPLC
Approx. Protein Concentrations: Total protein concentration 0.5 mg/ml after reconstitution
Protein Molecular Weight: 12.9 kDa based on 115 amino acid sequence
Endotoxin Level: <0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

More Information

UniProt: P40933 Related reagents
Entrez Gene: IL15 Related reagents
GO Terms: GO:0004871 signal transducer activity; GO:0005887 integral to plasma membrane; GO:0005125 cytokine activity; GO:0005126 cytokine receptor binding; GO:0005615 extracellular space; GO:0005624 membrane fraction; GO:0005634 nucleus; GO:0005768 endosome; GO:0005794 Golgi apparatus; GO:0006955 immune response; GO:0007267 cell-cell signaling; GO:0032740 positive regulation of interleukin-17 production; GO:0034105 positive regulation of tissue remodeling; GO:0050729 positive regulation of inflammatory response
Regulatory: For research purposes only

Applications of IL-15

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays		0.1ng/ml	10ng/ml

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.

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