IgG3

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Native Human IgG3

Product Type
Purified Protein
Isotype
IgG3
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5248-3004 ID 0.5 mg
Native Human IgG3 is a purified preparation og human IgG3 obtained from myeloma serum and urine. A single arc is observed using immunoelectophoresis against anti whole serum and anti human IgG3 antibodies. No reactivitiy is observed using inti IgG1, IgG2 or IgG4 by RID.

Product Details

Target Species
Human
Product Form
Purified immunoglobulin from human myeloma serum or urine - liquid
Preparation
Ion exchange chromatography, gel filtration and protein A chromatography, 0.2 µm filtered.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
0.1%EACA
0.01%Benzamidine
Purity
In IEP one arc was observed against anti-whole serum and anti-human IgG3. No reactivity was observed with anti IgG1, IgG2 or IgG4 by RID.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01860 Related reagents
Entrez Gene
IGHG3 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunodiffusion
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for IgG3

  1. Haberland, A. et al. (2016) Aptamer BC 007 – a broad spectrum neutralizer of pathogenic autoantibodies against G-protein-coupled receptors
    European Journal of Pharmacology. Jul 1 [Epub ahead of print]