Human IgG2/4 | AbD18705io
Recombinant Human IgG2/4 Kappa, clone AbD18705io (HCA337) was analysed under reducing, denaturing conditions (Lane 2) and non-reducing, denaturing conditions (Lane 3). Bands were visualized using Coomassie staining and the fully assembled antibody ((LC-HC)2), the heavy (HC) and light chains (LC) are indicated. The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand side of the image.
UV280 chromatogram of a size exclusion chromatography run of recombinant human IgG2/4 kappa, clone AbD18705io (HCA337). The larger peak corresponds to monomeric, fully assembled AbD18705io. The smaller peaks most likely contain dimers and oligomers.
Recombinant Human IgG2/4 kappa, clone AbD18705io, contains a kappa light chain and a hybrid heavy chain constant region combining sequences from IgG2 and IgG4.
This product is a recombinant fully human antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for studies using IgG2/4 CH hybrid antibodies.
The heavy chain constant region of Recombinant Human IgG2/4 kappa consists of a fusion construct:
CH1, Hinge and N-terminal of CH2 identical to human IgG2 constant region (IMGT allele IGHG2*01)
C-terminal of CH2 and CH3 identical to human IgG4 constant region (IMGT allele IGHG4*04)
IgG2 and IgG4 are the least effector potent immunoglobulin subclasses, and have been combined in the design of antibodies that block their target without the induction of effector functions. An example is the therapeutic monoclonal antibody drug eculizumab (Soliris), which is used clinically to block complement factor C5, and is approved to treat the rare diseases paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS).
View Human Anti-C5 Antibody (Eculizumab Biosimilar)
View the full range of recombinant fully human immunoglobulin isotype controls
The heavy chain constant region of Recombinant Human IgG2/4 kappa consists of a fusion construct:
CH1, Hinge and N-terminal of CH2 identical to human IgG2 constant region (IMGT allele IGHG2*01)
C-terminal of CH2 and CH3 identical to human IgG4 constant region (IMGT allele IGHG4*04)
IgG2 and IgG4 are the least effector potent immunoglobulin subclasses, and have been combined in the design of antibodies that block their target without the induction of effector functions. An example is the therapeutic monoclonal antibody drug eculizumab (Soliris), which is used clinically to block complement factor C5, and is approved to treat the rare diseases paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS).
View Human Anti-C5 Antibody (Eculizumab Biosimilar)
View the full range of recombinant fully human immunoglobulin isotype controls
Product Details
- Product Form
- Human IgG2/4 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Human IgG2/4
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti C5 (Eculizumab Biosimilar) | MCA6145 | E | 0.2 mg |
|
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
