Human IgG2/4 | AbD18705io

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Recombinant Human IgG2/4 Kappa

Product Type
Recombinant Protein
Clone
AbD18705io
Isotype
IgG2 Kappa
IgG4 Kappa
Product Code Applications Pack Size List Price Quantity
HCA337
Datasheet
E
SDS
0.1 mg loader

Recombinant Human IgG2/4 kappa, clone AbD18705io, contains a kappa light chain and a hybrid heavy chain constant region combining sequences from IgG2 and IgG4. This product is a recombinant fully human antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for studies using IgG2/4 CH hybrid antibodies.

The heavy chain constant region of Recombinant Human IgG2/4 kappa consists of a fusion construct:
CH1, Hinge and N-terminal of CH2 identical to human IgG2 constant region (IMGT allele IGHG2*01)
C-terminal of CH2 and CH3 identical to human IgG4 constant region (IMGT allele IGHG4*04)

IgG2 and IgG4 are the least effector potent immunoglobulin subclasses, and have been combined in the design of antibodies that block their target without the induction of effector functions. An example is the therapeutic monoclonal antibody drug eculizumab (Soliris), which is used clinically to block complement factor C5, and is approved to treat the rare diseases paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS).

View Human Anti-C5 Antibody (Eculizumab Biosimilar)

View the full range of recombinant fully human immunoglobulin isotype controls

Product Details

Product Form
Human IgG2/4 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgG2/4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human anti C5 (Eculizumab Biosimilar) MCA6145 E 0.2 mg loader

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