IgG1

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Human IgG1 Kappa

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP010 E FN* 1 mg

Product Details

Target Species
Human
Product Form
Purified protein - liquid
Preparation
Purified protein prepared from human myeloma serum by ion exchange chromatography, gel filtration and protein A chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
<0.1% EACA
<0.01% Benzamidine
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P01834 Related reagents
P01857 Related reagents
Entrez Gene
IGKC Related reagents
IGHG1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 1
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassette (EQU003) is suitable for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Application Based External Images

ELISA

Functional Assays

Product Specific References

References for IgG1

  1. Campbell, S. et al. (2006) Proinflammatory effects of TWEAK/Fn14 interactions in glomerular mesangial cells.
    J Immunol. 176:1889-98.
  2. Hershkovitz, O. et al. (2009) NKp44 receptor mediates interaction of the envelope glycoproteins from the West Nile and dengue viruses with NK cells.
    J Immunol. 183: 2610-21.
  3. Rosano, J.M. et al. (2009) A physiologically realistic in vitro model of microvascular networks.
    Biomed Microdevices. 11 (5): 1051-7.
  4. Arnon, T.I. et al. (2008) Harnessing soluble NK cell killer receptors for the generation of novel cancer immune therapy.
    PLoS One. 3(5): e2150.
  5. Ostrowitzki, S. et al. (2012) Mechanism of amyloid removal in patients with Alzheimer disease treated with gantenerumab.
    Arch Neurol. 69 (2): 198-207.
  6. Rosental, B. et al. (2011) Proliferating Cell Nuclear Antigen Is a Novel Inhibitory Ligand for the Natural Cytotoxicity Receptor NKp44.
    J Immunol. 187: 5693-702.
  7. Bloem, K. et al. (2013) DCIR interacts with ligands from both endogenous and pathogenic origin.
    Immunol Lett. pii: S0165-2478(13)00174-0.