IGFBP-7

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Recombinant Human IGFBP-7

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP178 E WB 25 µg
IGFBP-7 (insulin-like growth factor binding protein-7) is a secreted protein which belongs to a family of IGF-BPs which regulate the biological activities and distribution of the insulin-like growth factors (IGFs), through binding to IGF-I and IGF-II. IGFBP-7 is expressed in many tumour types and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.25 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the additiona of 0.1% BSA is recommended.
Preparation
Purified recombinant IGFBP-7 expressed in E.coli.
Buffer Solution
10mM Sodium Phosphate, pH6.8
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Molecular Weight
26.4kD (257 amino acid residues).
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
Q16270 Related reagents
Entrez Gene
IGFBP7 Related reagents
GO Terms
GO:0001558 regulation of cell growth
GO:0007155 cell adhesion
GO:0005520 insulin-like growth factor binding
GO:0005615 extracellular space
GO:0008285 negative regulation of cell proliferation
Regulatory
For research purposes only

Applications of IGFBP-7

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This recombinant protein may be used as a standard in an ELISA with AHP1282.
Western Blotting
This recombinant protein may be as a positive control in western blotting for AHP1282 and may be run under either reducing or non-reducing conditions.