IGFBP-7
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IGFBP-7 (insulin-like growth factor binding protein-7) is a secreted protein which belongs to a family of IGF-BPs which regulate the biological activities and distribution of the insulin-like growth factors (IGFs), through binding to IGF-I and IGF-II. IGFBP-7 is expressed in many tumour types and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitution
- Reconstitute with 0.25 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the additiona of 0.1% BSA is recommended. - Preparation
- Purified recombinant IGFBP-7 expressed in E.coli.
- Buffer Solution
- 10mM Sodium Phosphate, pH6.8
- Preservative Stabilisers
- None present
- Purity
- >98% by SDS PAGE and HPLC analysis.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Molecular Weight
- 26.4kD (257 amino acid residues).
- Endotoxin Level
- <1EU/ug
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 3 months from date of reconstitution.
More Information
- UniProt
- Q16270 Related reagents
- Entrez Gene
- IGFBP7 Related reagents
- GO Terms
- GO:0001558 regulation of cell growth
- GO:0007155 cell adhesion
- GO:0005520 insulin-like growth factor binding
- GO:0005615 extracellular space
- GO:0008285 negative regulation of cell proliferation
- Regulatory
- For research purposes only
Applications of IGFBP-7
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4ng/well | |
| Western Blotting | 1.5 | 3.0ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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