IGFBP-7

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Recombinant Human IGFBP-7

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP178 Datasheet	E WB SDS	25 µg	Log in		Get Quote

Summary
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IGFBP-7 (insulin-like growth factor binding protein-7) is a secreted protein which belongs to a family of IGF-BPs which regulate the biological activities and distribution of the insulin-like growth factors (IGFs), through binding to IGF-I and IGF-II. IGFBP-7 is expressed in many tumour types and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 0.25 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the additiona of 0.1% BSA is recommended.
Preparation: Purified recombinant IGFBP-7 expressed in E.coli.
Buffer Solution: 10mM Sodium Phosphate, pH6.8
Preservative Stabilisers: None present
Carrier Free: Yes
Purity: >98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 26.4 kDa (257 amino acid residues).
Endotoxin Level: <1EU/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 3 months from date of reconstitution

More Information

UniProt: Q16270
Entrez Gene: IGFBP7
GO Terms: GO:0001558 regulation of cell growth; GO:0007155 cell adhesion; GO:0005520 insulin-like growth factor binding; GO:0005615 extracellular space; GO:0008285 negative regulation of cell proliferation
Regulatory: For research purposes only

Applications of IGFBP-7

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		0.2	0.4ng/well
Western Blotting		1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This recombinant protein may be used as a standard in an ELISA with AHP1282.
Western Blotting: This recombinant protein may be as a positive control in western blotting for AHP1282 and may be run under either reducing or non-reducing conditions.

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