IGF-I
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Recombinant Human insulin-like growth factoor I is recombinant human IGF-1 producd in E.coli. It exists as a single non-glycosylated polypeptide chain containing 70 amino acids.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - liquid
- Preparation
- Purified recombinant human IGF-1 produced by E.coli
- Preservative Stabilisers
- None present
- Activity
- =>1x10(e)6 U/mg
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <= 1.0 ng/ml. - Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- 1.0 mg/ml
- Protein Molecular Weight
- 7.7 kDa
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Store at -70oC. For long term storage, the addition of a carrier protein is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05019
- Entrez Gene
- IGF1
- GO Terms
- GO:0006260 DNA replication
- GO:0002576 platelet degranulation
- GO:0005158 insulin receptor binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0005179 hormone activity
- GO:0006916 anti-apoptosis
- GO:0006928 cellular component movement
- GO:0007265 Ras protein signal transduction
- GO:0007517 muscle organ development
- GO:0008083 growth factor activity
- GO:0009441 glycolate metabolic process
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
- GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration
- GO:0014896 muscle hypertrophy
- GO:0014904 myotube cell development
- GO:0014911 positive regulation of smooth muscle cell migration
- GO:0046326 positive regulation of glucose import
- GO:0016942 insulin-like growth factor binding protein complex
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0034392 negative regulation of smooth muscle cell apoptosis
- GO:0035630 bone mineralization involved in bone maturation
- GO:0040014 regulation of multicellular organism growth
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
- GO:0045445 myoblast differentiation
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0045740 positive regulation of DNA replication
- GO:0045821 positive regulation of glycolysis
- GO:0045840 positive regulation of mitosis
- GO:0046579 positive regulation of Ras protein signal transduction
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0050679 positive regulation of epithelial cell proliferation
- GO:0051450 myoblast proliferation
- Regulatory
- For research purposes only
Applications of IGF-I
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Functional Assays |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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