Recombinant Human insulin-like growth factoor I is recombinant human IGF-1 producd in E.coli. It exists as a single non-glycosylated polypeptide chain containing 70 amino acids.
- Target Species
- Product Form
- Purified recombinant protein - liquid
- Purified recombinant human IGF-1 produced by E.coli
- Preservative Stabilisers
- None present
- =>1x10(e)6 U/mg
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <= 1.0 ng/ml.
- >98% by SDS PAGE
- Approx. Protein Concentrations
- 1.0 mg/ml
- Protein Molecular Weight
- 7.7 kDa
- Endotoxin Level
- < 0.1 ng/ug
- Store at -70oC. For long term storage, the addition of a carrier protein is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
- 18 months from date of despatch
- Entrez Gene
- GO Terms
insulin receptor binding
insulin-like growth factor receptor binding
cellular component movement
Ras protein signal transduction
muscle organ development
growth factor activity
glycolate metabolic process
positive regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of phosphatidylinositol 3-kinase cascade
satellite cell maintenance involved in skeletal muscle regeneration
myotube cell development
positive regulation of smooth muscle cell migration
positive regulation of glucose import
insulin-like growth factor binding protein complex
platelet alpha granule lumen
negative regulation of smooth muscle cell apoptosis
bone mineralization involved in bone maturation
regulation of multicellular organism growth
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of insulin-like growth factor receptor signaling pathway
positive regulation of osteoblast differentiation
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
positive regulation of glycolysis
positive regulation of mitosis
positive regulation of Ras protein signal transduction
positive regulation of fibroblast proliferation
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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