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IGF-I

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Recombinant Human Insulin-Like Growth Factor I

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
5345-0654
Datasheet
FN
SDS
50 µg loader

Recombinant Human insulin-like growth factoor I is recombinant human IGF-1 producd in E.coli. It exists as a single non-glycosylated polypeptide chain containing 70 amino acids.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - liquid
Preparation
Purified recombinant human IGF-1 produced by E.coli
Preservative Stabilisers
None present
Activity
=>1x10(e)6 U/mg
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <= 1.0 ng/ml.
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
7.7 kDa
Endotoxin Level
< 0.1 ng/ug

Storage Information

Storage
Store at -70oC. For long term storage, the addition of a carrier protein is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Guarantee
12 months from date of despatch

More Information

UniProt
P05019
Entrez Gene
IGF1
GO Terms
GO:0006260 DNA replication
GO:0002576 platelet degranulation
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0007265 Ras protein signal transduction
GO:0007517 muscle organ development
GO:0008083 growth factor activity
GO:0009441 glycolate metabolic process
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration
GO:0014896 muscle hypertrophy
GO:0014904 myotube cell development
GO:0014911 positive regulation of smooth muscle cell migration
GO:0046326 positive regulation of glucose import
GO:0016942 insulin-like growth factor binding protein complex
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0034392 negative regulation of smooth muscle cell apoptosis
GO:0035630 bone mineralization involved in bone maturation
GO:0040014 regulation of multicellular organism growth
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
GO:0045445 myoblast differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0045740 positive regulation of DNA replication
GO:0045821 positive regulation of glycolysis
GO:0045840 positive regulation of mitosis
GO:0046579 positive regulation of Ras protein signal transduction
GO:0048146 positive regulation of fibroblast proliferation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050679 positive regulation of epithelial cell proliferation
GO:0051450 myoblast proliferation
Regulatory
For research purposes only

Applications of IGF-I

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Product Specific References

References for IGF-I

  1. Fredolini, C. et al. (2020) Shotgun proteomics coupled to nanoparticle-based biomarker enrichment reveals a novel panel of extracellular matrix proteins as candidate serum protein biomarkers for early-stage breast cancer detection.
    Breast Cancer Res. 22 (1): 135.

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