IGF-I

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Recombinant Human Insulin-Like Growth Factor I

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
5345-0654 Datasheet	FN SDS	50 µg	Log in		Get Quote

Recombinant Human insulin-like growth factoor I is recombinant human IGF-1 producd in E.coli. It exists as a single non-glycosylated polypeptide chain containing 70 amino acids.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - liquid
Preparation: Purified recombinant human IGF-1 produced by E.coli
Preservative Stabilisers: None present
Activity: =>1x10(e)6 U/mg
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <= 1.0 ng/ml.
Purity: >98% by SDS PAGE
Approx. Protein Concentrations: 1.0 mg/ml
Protein Molecular Weight: 7.7 kDa
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Store at -70^oC. For long term storage, the addition of a carrier protein is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Guarantee: 12 months from date of despatch

More Information

UniProt: P05019
Entrez Gene: IGF1
GO Terms: GO:0006260 DNA replication; GO:0002576 platelet degranulation; GO:0005158 insulin receptor binding; GO:0005159 insulin-like growth factor receptor binding; GO:0005179 hormone activity; GO:0006916 anti-apoptosis; GO:0006928 cellular component movement; GO:0007265 Ras protein signal transduction; GO:0007517 muscle organ development; GO:0008083 growth factor activity; GO:0009441 glycolate metabolic process; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade; GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration; GO:0014896 muscle hypertrophy; GO:0014904 myotube cell development; GO:0014911 positive regulation of smooth muscle cell migration; GO:0046326 positive regulation of glucose import; GO:0016942 insulin-like growth factor binding protein complex; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030168 platelet activation; GO:0031093 platelet alpha granule lumen; GO:0034392 negative regulation of smooth muscle cell apoptosis; GO:0035630 bone mineralization involved in bone maturation; GO:0040014 regulation of multicellular organism growth; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway; GO:0045445 myoblast differentiation; GO:0045669 positive regulation of osteoblast differentiation; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0045740 positive regulation of DNA replication; GO:0045821 positive regulation of glycolysis; GO:0045840 positive regulation of mitosis; GO:0046579 positive regulation of Ras protein signal transduction; GO:0048146 positive regulation of fibroblast proliferation; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0050679 positive regulation of epithelial cell proliferation; GO:0051450 myoblast proliferation
Regulatory: For research purposes only

Applications of IGF-I

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IGF-I

Fredolini, C. et al. (2020) Shotgun proteomics coupled to nanoparticle-based biomarker enrichment reveals a novel panel of extracellular matrix proteins as candidate serum protein biomarkers for early-stage breast cancer detection.
Breast Cancer Res. 22 (1): 135.

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