Recombinant human lFN-α; A expressed in Escherichia coli.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified recombinant protein - liquid
- Prepared by a combination of ion exchange, hydrophobic interactions and size exclusion chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 8.96 x 107 units/ml
Activity is determined via cytopathic inhibition assay on MDBK cells.
- >95% by SDS PAGE
- Protein Molecular Weight
- 19.2 kD
- Endotoxin Level
- < 1.0 EU/ug
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- 3 months from date of despatch
- Entrez Gene
- GO Terms
response to virus
interferon-alpha/beta receptor binding
induction of apoptosis
type I interferon-mediated signaling pathway
regulation of type I interferon-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IFN Alpha A
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IFN Alpha A
Kamiya, T. et al. (2010) Increased caspase-2 activity is associated with induction of apoptosis in
IFN-beta sensitive melanoma cell lines.
J Interferon Cytokine Res 30: 349-57.
Mathieu, M.G. et al. (2014) The helicase HAGE prevents interferon-α-induced PML expression in ABCB5+ malignant melanoma-initiating cells by promoting the expression of SOCS1.
Cell Death Dis. 5:e1061.
Jinushi M et al. (2016) Measles Virus Genotype D Wild Strains Suppress Interferon-Stimulated Gene Expression More Potently than Laboratory Strains in SiHa Cells.
Viral Immunol. Apr 1. [Epub ahead of print]
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra