IFN Alpha A

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Recombinant Human Interferon Alpha A

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP107Z FN 5 X 10*6 Units/0.1ml
Recombinant human lFN-α; A expressed in Escherichia coli.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified recombinant protein - liquid
Preparation
Prepared by a combination of ion exchange, hydrophobic interactions and size exclusion chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Bovine Serum Albumin
Activity
8.5 x 107 units/ml

Activity is determined via cyopathic inhibition assay on MDBK cells.
Purity
>95% by SDS PAGE
Protein Molecular Weight
19.2kD
Endotoxin Level
< 1 EU/ug

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of despatch.

More Information

UniProt
P01563 Related reagents
Entrez Gene
IFNA2 Related reagents
GO Terms
GO:0009615 response to virus
GO:0007596 blood coagulation
GO:0005125 cytokine activity
GO:0005132 interferon-alpha/beta receptor binding
GO:0005615 extracellular space
GO:0006917 induction of apoptosis
GO:0006954 inflammatory response
GO:0007267 cell-cell signaling
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
Regulatory
For research purposes only

Applications of IFN Alpha A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IFN Alpha A

  1. Kamiya, T. et al. (2010) Increased caspase-2 activity is associated with induction of apoptosis in IFN-beta sensitive melanoma cell lines.
    J Interferon Cytokine Res 30: 349-57.
  2. Mathieu, M.G. et al. (2014) The helicase HAGE prevents interferon-α-induced PML expression in ABCB5+ malignant melanoma-initiating cells by promoting the expression of SOCS1.
    Cell Death Dis. 5:e1061.
  3. Jinushi M et al. (2016) Measles Virus Genotype D Wild Strains Suppress Interferon-Stimulated Gene Expression More Potently than Laboratory Strains in SiHa Cells.
    Viral Immunol. Apr 1. [Epub ahead of print]