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Fractalkine

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Recombinant Human Fractalkine

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP113
Datasheet
FN
MSDS
20 µg loader

Human Fractalkine is a member of the CX3C family of chemokines which has both a chemokine and a mucin domain.PHP113 is chemotactic for human monocytes.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein prepared from E.Coli
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>99% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml
Protein Molecular Weight
8.5kDa (76 amino acid sequence)
Endotoxin Level
< 0.1 ng/mg

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC or at -20oC if preferred.

This product should be stored undiluted

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
3 months from date of reconstitution

More Information

UniProt
P78423
Entrez Gene
CX3CL1
GO Terms
GO:0005886 plasma membrane
GO:0006952 defense response
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0009986 cell surface
GO:0019221 cytokine-mediated signaling pathway
GO:0050729 positive regulation of inflammatory response
GO:0050902 leukocyte adhesive activation
GO:0051041 positive regulation of calcium-independent cell-cell adhesion
Regulatory
For research purposes only

Applications of Fractalkine

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 5ng/ml 10ng/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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