FGF Acidic

100% Secure

Recombinant Human FGF Acidic

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP031 FN 50 µg
Human FGF acidic, also known as FGF 1 (fibroblast growth factor 1), is a member of the heparin-binding growth factor family, widely distributed in the peripheral and central nervous systems.

FGF 1 acts as a potent neurotrophic factor, playing a key role during prenatal development and postnatal growth/regeneration and is dependent upon interactions with heparin sulfate (HS) proteoglycans located on cell surfaces and in the extracellular matrix, and the four high affinity tyrosine kinase receptors, designated FGFR-1,-2,-3 and -4.

Bioactivity was determined by the dose-dependent stimulation of thymidine uptake by FGF receptor-expressing BaF3 cells. The ED50 is <10ng/ml.

Product Details

Target Species
Human
Product Form
Purified - lyophilised
Reconstitution
Reconstitute with 50ul of 5mM Sodium Phosphate, pH 8.0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Preparation
Recombinant protein expressed in E. coli.
Preservative Stabilisers
None present
Activity
1 x 105 units/mg
Purity
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
1 mg/ml after reconstitution
Protein Molecular Weight
15.8 kDa (140 Amino Acid Sequence)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P05230 Related reagents
Entrez Gene
FGF1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0005829 cytosol
GO:0005104 fibroblast growth factor receptor binding
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010740 positive regulation of intracellular protein kinase cascade
GO:0034605 cellular response to heat
GO:0045542 positive regulation of cholesterol biosynthetic process
GO:0048155 S100 alpha binding
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of FGF Acidic

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.