FGF 8

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Recombinant Human FGF 8

Product Type: Recombinant Protein
Specificity: FGF 8

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PHP130 Datasheet Datasheet SDS SDS	FN	25 µg		Log in	Get Quote
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Recombinant human FGF8 is an E. coli-derived protein corresponding to amino acids 23-215 of human fibroblast growth factor 8. FGF8 is a secreted peptide that promotes cell growth in an autocrine manner.

The peptide sequence contains an initiator methionine at the N-terminus and is homologous to the murine, canine and bovine sequences.

PHP130 has an ED₅₀ of <0.5ng/ml as determined by dose-dependent thymidine uptake in BaF3 cells expressing FGF receptors.

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 0.25 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% Bovine Serum Albumin is recommended.
Preparation: Recombinant protein expressed in Escherichia coli
Source: E.coli
Preservative Stabilisers: None present.
Carrier Free: Yes
Activity: 2 x 10⁶ units/mg
Purity: >95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight: 22.4 kD (193 amino acid sequence)
Endotoxin Level: < 0.1 ng/ug
Regulatory: For research purposes only
Guarantee: 3 months from date of reconstitution

Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays		0.1ng/ml	20ng/ml

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.

Synonyms: Fibroblast Growth Factor 8
UniProt: P55075
Entrez Gene: FGF8
GO Terms: GO:0001656 metanephros development; GO:0001823 mesonephros development; GO:0005576 extracellular region; GO:0005105 type 1 fibroblast growth factor receptor binding; GO:0005111 type 2 fibroblast growth factor receptor binding; GO:0005615 extracellular space; GO:0007369 gastrulation; GO:0008083 growth factor activity; GO:0008284 positive regulation of cell proliferation; View More GO Terms; GO:0008286 insulin receptor signaling pathway; GO:0008406 gonad development; GO:0008543 fibroblast growth factor receptor signaling pathway; GO:0042476 odontogenesis; GO:0051781 positive regulation of cell division; GO:0060348 bone development; GO:0060563 neuroepithelial cell differentiation; GO:0071542 dopaminergic neuron differentiation