FGF 8

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Recombinant Human FGF 8

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP130 FN 25 µg
Recombinant human FGF8 is an E. coli-derived protein corresponding to amino acids 23-215 of human fibroblast growth factor 8. FGF8 is a secreted peptide that promotes cell growth in an autocrine manner.

The peptide sequence contains an initiator methionine at the N-terminus and is homologous to the murine, canine and bovine sequences.

PHP130 has an ED50 of <0.5ng/ml as determined by dose-dependent thymidine uptake in BaF3 cells expressing FGF receptors.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.25ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% Bovine Serum Albumin is recommended.
Preparation
Recombinant protein expressed in Escherichia coli
Preservative Stabilisers
None present.
Activity
2 x 106 units/mg
Purity
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution
Protein Molecular Weight
22.4kD (193 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P55075 Related reagents
Entrez Gene
FGF8 Related reagents
GO Terms
GO:0001656 metanephros development
GO:0001823 mesonephros development
GO:0005576 extracellular region
GO:0005105 type 1 fibroblast growth factor receptor binding
GO:0005111 type 2 fibroblast growth factor receptor binding
GO:0005615 extracellular space
GO:0007369 gastrulation
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0008406 gonad development
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0042476 odontogenesis
GO:0051781 positive regulation of cell division
GO:0060348 bone development
GO:0060563 neuroepithelial cell differentiation
GO:0071542 dopaminergic neuron differentiation
Regulatory
For research purposes only

Applications of FGF 8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 0.1ng/ml 20ng/ml
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.