Cystatin C

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Recombinant Human Cystatin C

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP202 E 0.1 mg
Recombinant Human cystatin C is a prpparation of cystatin C madein E. coli. Cystatin C is a member of the type 2 cystatin superfamily. It is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsins), and pathogenic. Cystatin C is a 13kDa secreted protein which is found in high concentrations in biological fluids and is thought to be expressed by all human nucleated cells.

Changes in cystatin C have been documented in a number of cardiovascular diseases, including atherosclerosis, amyloid angiopathy and myocardial infarction. Its low molecular weight also means that Cystatin C can be used as a marker of renal failure.

Recombinant Human cystatin C (PHP202) is a single polypeptide chain corresponding to mature secreted protein with an additional Met residue at the N-terminal.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with deionized water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant Cystatin C expressed in E.coli
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P01034 Related reagents
Entrez Gene
CST3 Related reagents
GO Terms
GO:0001540 beta-amyloid binding
GO:0002020 protease binding
GO:0006952 defense response
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0010703 negative regulation of histolysis
GO:0010711 negative regulation of collagen catabolic process
GO:0010716 negative regulation of extracellular matrix disassembly
GO:0043206 fibril organization
GO:0060311 negative regulation of elastin catabolic process
GO:0060313 negative regulation of blood vessel remodeling
Regulatory
For research purposes only

Applications of Cystatin C

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP202 may be used as a standard in ELISA applications with either MCA2639, MCA2675 or MCA2676.