Collagen V

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Native Human Collagen V

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0190 E 0.1 mg

Product Details

Target Species
Human
Product Form
Purified protein from human placenta - lyophilised
Reconstitution
Use 0.1 M acetic acid, pH 3.0. The dissolved collagen retains immunogenic properties. Thermal denaturation (5 min at 100ÂșC) converts dissolved collagen to gelatin.
Preparation
Purified by acetic acid extraction after pepsin treatment followed by serial salt precipitations and ion-exchange chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurements and by reaction with anti-collagen type specific monoclonal antibodies.
Buffer Solution
Essentially salt free
Preservative Stabilisers
None present.
Purity
SDS PAGE: >95%

Contaminants:
Human Collagen Type I<1%
Human Collagen Type III<1%
Human Collagen Type IV<5%
Human Collagen Type VI<1%

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P20908 Related reagents
P05997 Related reagents
P25940 Related reagents
Entrez Gene
COL5A2 Related reagents
COL5A1 Related reagents
COL5A3 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005178 integrin binding
GO:0005588 collagen type V
GO:0007411 axon guidance
GO:0008201 heparin binding
GO:0030199 collagen fibril organization
GO:0032964 collagen biosynthetic process
GO:0035313 wound healing, spreading of epidermal cells
GO:0043206 fibril organization
GO:0043394 proteoglycan binding
GO:0043588 skin development
GO:0045112 integrin biosynthetic process
GO:0048407 platelet-derived growth factor binding
GO:0048592 eye morphogenesis
GO:0005201 extracellular matrix structural constituent
GO:0005518 collagen binding
Regulatory
For research purposes only

Applications of Collagen V

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Collagen V

  1. Glanville, R.W. et al. (1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains
    Eur. J. Biochem., 95: 383-389
  2. Sage, H. & Bornstein, P. (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen
    Biochemistry. 18: 3815-3822
  3. Klasson, S. C. et al. (1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta
    Coll. Rel. Res. 6: 397-402