CD54

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Recombinant Human CD54

Product Type: Recombinant Protein
Specificity: CD54

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PHP250 Datasheet Datasheet SDS SDS	E FN WB	50 µg		Log in	Get Quote
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CD54, otherwise known as ICAM-1, is a widely expressed type I integral membrane glycoprotein and member of the immunoglobulin superfamily, which acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, and is the major receptor for human rhinovirus. CD54 is highly expressed on activated endothelial cells and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress. Levels of soluble CD54 in serum, can be potentially used as an indication of infection, inflammation and certain tumours, and as a target for immunosuppression in transplantation.

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified recombinant CD54 expressed in CHO cells
Source: CHO cells
Buffer Solution: 10mM Sodium Phosphate
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: CD54 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at a CD54 concentration of 25ng/ml.
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 49.5 kDa (453 amino acid residues)
Endotoxin Level: < 1.0 EU/ug
Regulatory: For research purposes only
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PHP250 may be used as a standard in ELISA applications involving AHP2183 or AHP2183B.
Western Blotting: PHP250 may be used as a positive control for western blotting applications involving AHP2183 or AHP2183B.

Synonyms: ICAM-1
UniProt: P05362
Entrez Gene: ICAM1
GO Terms: GO:0007157 heterophilic cell-cell adhesion; GO:0001910 regulation of leukocyte mediated cytotoxicity; GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; GO:0002693 positive regulation of cellular extravasation; GO:0004888 transmembrane receptor activity; GO:0005887 integral to plasma membrane; GO:0005178 integrin binding; GO:0005615 extracellular space; GO:0007159 leukocyte cell-cell adhesion; View More GO Terms; GO:0022614 membrane to membrane docking; GO:0046813 virion attachment, binding of host cell surface receptor; GO:0050776 regulation of immune response; GO:0050900 leukocyte migration; GO:0051856 adhesion to symbiont; GO:0060333 interferon-gamma-mediated signaling pathway