CD54

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Recombinant Human CD54

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP250 E FN WB 50 µg
CD54, otherwise known as ICAM-1, is a widely expressed type I integral membrane glycoprotein and member of the immunoglobulin superfamily, which acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, and is the major receptor for human rhinovirus. CD54 is highly expressed on activated endothelial cells and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress. Levels of soluble CD54 in serum, can be potentially used as an indication of infection, inflammation and certain tumours, and as a target for immunosuppression in transplantation.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant CD54 expressed in CHO cells
Buffer Solution
10mM Sodium Phosphate
Preservative Stabilisers
None present
Activity
CD54 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at a CD54 concentration of 25ng/ml.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Molecular Weight
49.5kD (453 amino acid residues)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P05362 Related reagents
Entrez Gene
ICAM1 Related reagents
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001910 regulation of leukocyte mediated cytotoxicity
GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
GO:0002693 positive regulation of cellular extravasation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0007159 leukocyte cell-cell adhesion
GO:0022614 membrane to membrane docking
GO:0046813 virion attachment, binding of host cell surface receptor
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
GO:0051856 adhesion to symbiont
GO:0060333 interferon-gamma-mediated signaling pathway
Regulatory
For research purposes only

Applications of CD54

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP250 may be used as a standard in ELISA applications involving AHP2183 or AHP2183B.
Western Blotting
PHP250 may be used as a positive control for western blotting applications involving AHP2183 or AHP2183B.