CD253

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Recombinant Human CD253

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP125 E FN WB 50 µg
Recombinant human CD253 is a 19.6 kDa protein comprised of 168 amino acids which correspond to the TNF-homologous region of the extracellular domain of human TRAIL/ApoL2.

TNF-related apoptosis-inducing ligand (TRAIL) is expressed in many tissues including spleen, lung and prostate (Wiley et al, 1995) and is capable of inducing apoptosis in a tumour specific manner (Walczak et al, 1997). Apoptosis is initiated by the binding of the two death receptors DR4 and DR5 which initiate cell death signalling via the caspase cascade. There are however two decoy receptors DcR1 and DcR2 which competitively bind to CD253 but do not initiate apoptosis (Allen and El-Deiry, 2012).

A potential use for TRAIL as an anti-tumor therapeutic agent has been identified in numerous studies, demonstrating tumoricidal activity in approximately two thirds of tumor cell lines tested (Griffith et al, 1999).

Recombinant human CD253 is the immunogen used to produce Rabbit anti human CD253 (AHP1215).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water. For extended storage, the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution.
Protein Molecular Weight
19.6kD (169 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P50591 Related reagents
Entrez Gene
TNFSF10 Related reagents
GO Terms
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0046872 metal ion binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006919 activation of caspase activity
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008624 induction of apoptosis by extracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
Regulatory
For research purposes only

Applications of CD253

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.2 20ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
Recombinant human CD253 may be used as a standard for ELISA applications with anti human CD253 (purified)
Western Blotting
Recombinant human CD253 may be used as the positive control for western blotting applications with anti human CD253 (purified)

Product Specific References

References for CD253

  1. Bumdelger, B. et al. (2016) Osteoprotegerin Prevents Development of Abdominal Aortic Aneurysms.
    PLoS One. 11 (1): e0147088.
  2. Kamata, R. et al. (2016) EPA Prevents the Development of Abdominal Aortic Aneurysms through Gpr-120/Ffar-4.
    PLoS One. 11 (10): e0165132.