CD154

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Recombinant Human CD154

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP126 FN WB 10 µg
Recombinant Human CD154 is an E.coliderived protein corresponding to human CD154, also known as CD40 ligand, CD40L or TRAP. CD154 is a membrane protein expressed by activated T cells. Binding of CD154 to its ligand (CD40) on B cells induces B cell proliferation and immunoglobulin class switching.

This preparation of Recombinant Human CD154 comprises the receptor binding TNF-like domain of human CD154.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 10ul 5-10mM sodium phosphate pH7.5. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in E.Coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and HPLC sequence analysis
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
16.3kDa (149 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P29965 Related reagents
Entrez Gene
CD40LG Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0030168 platelet activation
GO:0032735 positive regulation of interleukin-12 production
GO:0042100 B cell proliferation
GO:0045190 isotype switching
Regulatory
For research purposes only

Applications of CD154

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Western Blotting 0.05 ug 0.5 ug
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.