CD120b
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Recombinant human CD120b is an 18.9 kDa peptide comprised of 174 amino acids representing the soluble form of CD120b. Soluble human CD120b, otherwise known as sTNF receptor type II (sTNFRII), is a member of the tumour necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE.
CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFRII acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor.
CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFRII acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 0.2 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified recombinant CD120b expressed in E.coli
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- None present
- Activity
- Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.125 µg/ml.
- Purity
- >98% by SDS PAGE and HPLC
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution.
- Protein Molecular Weight
- 18.9 kDa (174 amino acid residues)
- Endotoxin Level
- <1 EU/ug
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
More Information
- UniProt
- P20333 Related reagents
- Entrez Gene
- TNFRSF1B Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005031 tumor necrosis factor receptor activity
- GO:0045121 membrane raft
- Regulatory
- For research purposes only
Applications of CD120b
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4 ng/well | |
| Functional Assays | 0.125ug/ml | ||
| Western Blotting | 1.5 | 3.0 ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Recombinant human CD120b may be used as a standard for ELISA applications with either a purified human CD120b antibody (AHP1833) or a biotinylated human CD120b antibody (AHP1833B).
- Western Blotting
- Recombinant human CD120b may be used as the positive control in Western Blotting applications with either a purified human CD120b antibody (AHP1833) or a biotinylated human CD120b antibody (AHP1833B).
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Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Human CD120b | AHP1833 | 0.1 mg | E FN IF WB | ||
| Rabbit anti Human CD120b:Biotin | AHP1833B | 50 µg | E WB |
