CD120b

Recombinant Human CD120b

Product Type
Recombinant Protein
Specificity
CD120b

Product Code Applications Pack Size List Price Your Price Qty
PHP215
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 20 µg loader
List Price Your Price
loader

Recombinant human CD120b is an 18.9 kDa peptide comprised of 174 amino acids representing the soluble form of CD120b. Soluble human CD120b, otherwise known as sTNF receptor type II (sTNFRII), is a member of the tumour necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE.

CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFRII acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.2 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant CD120b expressed in E.coli
Source
E.coli
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.125 µg/ml.
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
0.1 mg/ml after reconstitution.
Protein Molecular Weight
18.9 kDa (174 amino acid residues)
Endotoxin Level
< 1.0 EU/ug
Regulatory
For research purposes only
Guarantee
3 months from date of reconstitution

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4 ng/well
Functional Assays 0.125ug/ml
Western Blotting 1.5 3.0 ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human CD120b may be used as a standard for ELISA applications with either a purified human CD120b antibody (AHP1833) or a biotinylated human CD120b antibody (AHP1833B).
Western Blotting
Recombinant human CD120b may be used as the positive control in Western Blotting applications with either a purified human CD120b antibody (AHP1833) or a biotinylated human CD120b antibody (AHP1833B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Human CD120b AHP1833 E FN IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit anti Human CD120b
Rabbit anti Human CD120b:Biotin AHP1833B E WB 50 µg loader
List Price Your Price
loader
Description Rabbit anti Human CD120b:Biotin

Synonyms
TNF-R2
UniProt
P20333
Entrez Gene
TNFRSF1B
GO Terms
GO:0005886 plasma membrane
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005031 tumor necrosis factor receptor activity
GO:0045121 membrane raft
View more products with CD120 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products