CCL17
Recombinant Human CCL17 (PHP295) was analyzed under reducing denaturing conditions (Lane 2). The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand of the image. Bands were visualized using Coomassie stains.
Recombinant Human CCL17
- Product Type
- Recombinant Protein
- Specificity
- CCL17
Antibody Quality and Performance Guarantee
| Recombinant Human CCL17 CCL17 also known as TARC and SCYA17 is a C-C chemokine highly expressed by the thymus and other cell types, including fibroblasts (Li et al. 2019). CCL17 induces and enhances a broad spectrum of immune reactions, including some contact hypersensitivity reactions, allograft rejections, inflammatory bowel disease, atopic dermatitis and atherosclerosis (Scheu et al. 2017). Therapeutic use of antibodies to neutralize CCL17 can block influx of T cells and ameliorate the impact of various diseases (Teplyakov et al. 2017). Upregulation of CCL17 has been reported in breast cancer, and predicts a decrease in overall survival for patients with breast cancer (Li et al. 2019). This recombinant Human CCL17 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human CCL17 antibody, clone I02-2A4 (MCA6180GA) as the capture antibody and biotinylated Mouse anti Human CCL17 antibody, clone I11-2A1 (MCA6181B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex. - Preparation
- Recombinant protein expressed in E. coli and purified by ion exchange chromatography
- Source
- E.coli
- Buffer Solution
- 50 mM Tris-HCl, pH 8.0, 0.15 M Sodium Chloride
- Preservative Stabilisers
- 1.0% Trehalose
- Purity
- ≥90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
- Approx. Protein Concentrations
- 100 μg/ml after reconstitution
- Protein Molecular Weight
- 8.1 kDa
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human CCL17 | MCA6180GA | IY | 0.1 mg |
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| Description | Mouse anti Human CCL17 | ||||||
| Mouse anti Human CCL17:Biotin | MCA6181B | IY | 0.1 mg |
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| Description | Mouse anti Human CCL17:Biotin | ||||||
- UniProt
- Q92583
- Entrez Gene
- CCL17
- GO Terms
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0007267 cell-cell signaling
- GO:0007275 multicellular organismal development
- GO:0008009 chemokine activity
PHP295
100005637
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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