Annexin V

100% Secure

Recombinant Human Annexin V

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP186 IA 30 µg
Recombinant Human Annexin V is a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis.

Detection of PS using Annexin V provides a reliable diagnostic tool for the indication of early programmed cell death. Bio-Rad recommend the use of Annexin V kits for this purpose.

Annexin V has also been identified as an inhibitor of prothrombin activation in the blood coagulation cascade, and the presence of antibodies to Annexin V are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water at 30oC for 5 minutes.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant Annexin V expressed in E.coli.
Buffer Solution
20mM Phosphate Buffer
0.02% Tween 80
130mM Arginine HCl.
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE/HPLC analysis
Approx. Protein Concentrations
Total protein concentration 30ug/ml after reconstitution
Molecular Weight
35.8kD

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of reconstitution.

More Information

UniProt
P08758 Related reagents
Entrez Gene
ANXA5 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation
Regulatory
For research purposes only

Applications of Annexin V

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
In vitro Assay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.