Annexin V
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Recombinant Human Annexin V is a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis.
Detection of PS using Annexin V provides a reliable diagnostic tool for the indication of early programmed cell death. Bio-Rad recommend the use of Annexin V kits for this purpose.
Annexin V has also been identified as an inhibitor of prothrombin activation in the blood coagulation cascade, and the presence of antibodies to Annexin V are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
Detection of PS using Annexin V provides a reliable diagnostic tool for the indication of early programmed cell death. Bio-Rad recommend the use of Annexin V kits for this purpose.
Annexin V has also been identified as an inhibitor of prothrombin activation in the blood coagulation cascade, and the presence of antibodies to Annexin V are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitution
- Reconstitute with 1.0ml distilled water at 30oC for 5 minutes.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified recombinant Annexin V expressed in E.coli.
- Buffer Solution
- 20mM Phosphate Buffer
0.02% Tween 80
130mM Arginine HCl. - Preservative Stabilisers
- None present
- Purity
- >98% by SDS PAGE/HPLC analysis
- Approx. Protein Concentrations
- Total protein concentration 30ug/ml after reconstitution
- Molecular Weight
- 35.8kD
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 6 months from date of reconstitution.
More Information
- UniProt
- P08758 Related reagents
- Entrez Gene
- ANXA5 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0007596 blood coagulation
- GO:0004859 phospholipase inhibitor activity
- GO:0007165 signal transduction
- GO:0005544 calcium-dependent phospholipid binding
- GO:0005737 cytoplasm
- GO:0006916 anti-apoptosis
- GO:0050819 negative regulation of coagulation
- Regulatory
- For research purposes only
Applications of Annexin V
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| In vitro Assay |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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