Alkaline Phosphatase
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Native Human alkaline phosphatase is a liquid preparation of purified alkaline phosphatase derived from human placenta. Alkaline phosphatases are ubiquitous enzymes, occuring an species from bacteria to higher vertebrates, including man. They catalyse the hydrolysis of phosphate monoesters at basic pH values. In man there are four forms of alkaline phosphatase depending on tissue expression patterns. The four types are 'intestinal ALP', 'Placental ALP', 'germ cell ALP' and 'tissue non-specific ALP', also known as liver/ bone/ kidney ALP.
Product Details
- Target Species
- Human
- Product Form
- Purified AP from human placenta - liquid
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- None present.
- Activity
- 1069U/mg solid
1000U/ml One unit liberates one micromole of p-nitrophenol per minute at 37°C, pH 9.8 in diethanolamine buffer - Purity
- Biuret: 95%
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05187
- Entrez Gene
- ALPP
- GO Terms
- GO:0005886 plasma membrane
- GO:0004035 alkaline phosphatase activity
- GO:0016021 integral to membrane
- GO:0046872 metal ion binding
- GO:0009986 cell surface
- GO:0031225 anchored to membrane
- Regulatory
- For research purposes only
Applications of Alkaline Phosphatase
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Alkaline Phosphatase
-
Chrysanthopoulou, A. et al. (2017) Interferon lambda1/IL-29 and inorganic polyphosphate are novel regulators of neutrophil-driven thromboinflammation.
J Pathol. 243 (1): 111-22.
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