Alkaline Phosphatase

Native Human Alkaline Phosphatase

Product Type
Purified Protein
Specificity
Alkaline Phosphatase

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0300-2304
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Native Human alkaline phosphatase is a liquid preparation of purified alkaline phosphatase derived from human placenta. Alkaline phosphatases are ubiquitous enzymes, occuring an species from bacteria to higher vertebrates, including man. They catalyse the hydrolysis of phosphate monoesters at basic pH values. In man there are four forms of alkaline phosphatase depending on tissue expression patterns. The four types are 'intestinal ALP', 'Placental ALP', 'germ cell ALP' and 'tissue non-specific ALP', also known as liver/ bone/ kidney ALP.

Target Species
Human
Product Form
Purified AP from human placenta - liquid
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
None present.
Activity
1069U/mg solid
1000U/ml

One unit liberates one micromole of p-nitrophenol per minute at 37°C, pH 9.8 in diethanolamine buffer
Purity
Biuret: 95%
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for Alkaline Phosphatase

  1. Chrysanthopoulou, A. et al. (2017) Interferon lambda1/IL-29 and inorganic polyphosphate are novel regulators of neutrophil-driven thromboinflammation.
    J Pathol. 243 (1): 111-22.

Synonyms
AP
UniProt
P05187
Entrez Gene
ALPP
GO Terms
GO:0005886 plasma membrane
GO:0004035 alkaline phosphatase activity
GO:0016021 integral to membrane
GO:0046872 metal ion binding
GO:0009986 cell surface
GO:0031225 anchored to membrane