Native Human alkaline phosphatase is a liquid preparation of purified alkaline phosphatase derived from human placenta. Alkaline phosphatases are ubiquitous enzymes, occuring an species from bacteria to higher vertebrates, including man. They catalyse the hydrolysis of phosphate monoesters at basic pH values. In man there are four forms of alkaline phosphatase depending on tissue expression patterns. The four types are 'intestinal ALP', 'Placental ALP', 'germ cell ALP' and 'tissue non-specific ALP', also known as liver/ bone/ kidney ALP.
- Target Species
- Product Form
- Purified AP from human placenta - liquid
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- None present.
- 1069U/mg solid
1000U/mlOne unit liberates one micromole of p-nitrophenol per minute at 37ºC, pH 10.15 in Tris buffer
- Biuret: 95%
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
alkaline phosphatase activity
integral to membrane
metal ion binding
anchored to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Alkaline Phosphatase
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Alkaline Phosphatase
Chrysanthopoulou, A. et al. (2017) Interferon lambda1/IL-29 and inorganic polyphosphate are novel regulators of neutrophil-driven thromboinflammation.
J Pathol. 243 (1): 111-22.