TNF Alpha
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Recombinant Horse TNF Alpha
- Product Type
- Recombinant Protein
- Specificity
- TNF Alpha
Antibody Quality and Performance Guarantee
| Equine Tumour Necrosis Factor Alpha (TNFα) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response. Equine Tumour Necrosis Factor Alpha has an ED50 < 0.05ng/ml, determined by measuring the cytolytic effect of recombinant equine TNFα on murine WEHI-13VAR-164 cells in the presence of actinomycin D. |
- Target Species
- Horse
- Product Form
- Purified Recombinant Protein- lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water. Further dilutions should be made in a buffer containing carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add azide. - Preparation
- Recombinant protein expressed in pichia pastoris
- Source
- Pichia pastoris
- Preservative Stabilisers
- None present
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 17.1kDa
- Endotoxin Level
- 0.15 EU/ug
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Functional Assays |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P29553
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
View more products with TNF ALPHA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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