IL-8

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Recombinant Horse Interleukin-8

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PEP005 FN 20 µg
Equine Interleukin 8
This preparation of Equine Interleukin 8 has an ED50 in the range 20-25 ng/ml determined by studies of the induction of equine IL-8 on neutrophil migration in vitro.

Product Details

Target Species
Horse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water. Further dilutions should be made in a buffer containing carrier protein.

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
8.5kDa.

Storage Information

Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
O62812 Related reagents
Entrez Gene
IL8 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of IL-8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Application Based External Images

Functional Assays

Product Specific References

References for IL-8

  1. Van de Walle, G.R.et al. (2009) Analysis of the herpesvirus chemokine-binding glycoprotein G residues essential for chemokine binding and biological activity.
    J Biol Chem. 284: 5968-76.
  2. Van de Walle, G.R.et al. (2007) Herpesvirus chemokine-binding glycoprotein G (gG) efficiently inhibits neutrophil chemotaxis in vitro and in vivo. PubMed PMID:
    J Immunol. 179: 4161-9.