Cholesterol Esterase

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Native Candida rugosa Cholesterol Esterase

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2069-9954 E FN 500 Units
Native Candida rugosa cholesterol esterase is a preparation of purified cholesterol esterase from the yeast Candida rugosa. Cholesterol esterase is an enzyme that catalyses the hydrolysis of cholesterol and some other sterol esters to liberate cholesterol plus a fatty acid anion.

Product Details

Target Species
Fungal
Product Form
Purified enzyme from Candida rugosa - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Activity
6 U/mg
One unit catalyses the hydrolysis of one micromole of cholestrol ester per minute in the presence of Sodium cholate at 37ºC, pH 6.7
Purity
Biuret: 90%
Approx. Protein Concentrations
100 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P32947 Related reagents
GO Terms
GO:0004806 triglyceride lipase activity
GO:0008203 cholesterol metabolic process
GO:0016042 lipid catabolic process
Regulatory
For research purposes only

Applications of Cholesterol Esterase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.