ECGS

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Endothelial Cell Growth Supplement

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4110-5004 TC 50 mg
Endothelial Cell Growth Supplement (ECGS), derived from bovine hypothlalmus is a beneficial media supplement for use in the following:

  • Vascular endothelial cells (human and animal), at a concentration of 100-300 μg/ml in serum supplemented media.

  • Hybridoma cell cloning, at a concentration of 25-150 μg/ml in serum supplemented media.

  • Balb/c-3T3 cells, at a concentration of 50-200 μg/ml in 5% serum supplemented media.
  • Product Details

    Product Form
    Purified Protein - lyophilised
    Reconstitution
    Use 5-10 ml of sterile physiological saline. Mix gently and transfer to a sterile, screw cap culture tube. Dilute to a final concentration of 50-300 µg/ml in the appropriate serum supplemented media. Solution may be filtered on low protein-binding membranes if necessary.
    Preparation
    0.1 micron filtered.
    Source
    Purified endothelial mitogen from bovine hypothalamus.
    Buffer Solution
    Phosphate buffered saline, pH 7.4
    Preservative Stabilisers
    See Buffer solution.
    Purity
    Microorganisms - not detected.
    Mycoplasma - not detected.

    Storage Information

    Storage
    Lyophilised product is stable for 6 months at 4ºC.
    Solutions are stable for 2 weeks at 4ºC or 1 month at -20ºC. Storage in frost-free freezers is not recommended.
    This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    Shelf Life
    6 months from date of despatch.

    More Information

    Regulatory
    For research purposes only

    Applications of ECGS

    This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    Application Name Verified Min Dilution Max Dilution
    Tissue Culture
    Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

    Useful Reagents Available

    Description Product Code Pack Size Applications List Price Quantity
    Mycoplasma Removal Agent BUF035 5 ml TC
    Mouse Monoclonal Antibody Isotyping Test Kit MMT1 10 Tests IS
    Rat Monoclonal Antibody Isotyping Test Kit RMT1 10 Tests IS

    Product Specific References

    References for ECGS

    1. Baker, M. et al. (2013) Stromal Claudin14-Heterozygosity, but Not Deletion, Increases Tumour Blood Leakage without Affecting Tumour Growth.
      PLoS One. 8: e62516.
    2. Browne, A. et al. (2015) Pharmacological Inhibition of β3 Integrin Reduces the Inflammatory Toxicities Caused by Oncolytic Adenovirus without Compromising Anticancer Activity.
      Cancer Res. 75 (14): 2811-21.
    3. Lemaire, M. et al. (2015) Arsenic Exposure Increases Monocyte Adhesion to the Vascular Endothelium, a Pro-Atherogenic Mechanism.
      PLoS One. 10 (9): e0136592.
    4. Dieterich, L.C. et al. (2017) Tumor-Associated Lymphatic Vessels Upregulate PDL1 to Inhibit T-Cell Activation.
      Front Immunol. 8: 66.
    5. Gittens, B.R. et al. (2017) Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation.
      J Immunol. 198 (11): 4458-69.

    Further Reading

    1. Pintus, C. et al. (1983) Endothelial cell growth supplement: a cell cloning factor that promotes the growth of monoclonal antibody producing hybridoma cells.
      J Immunol Methods. 61 (2): 195-200.