Endothelial Cell Growth Supplement (ECGS), derived from bovine hypothlalmus is a beneficial media supplement for use in the following:
Vascular endothelial cells (human and animal), at a concentration of 100-300 μg/ml in serum supplemented media. Hybridoma cell cloning, at a concentration of 25-150 μg/ml in serum supplemented media. Balb/c-3T3 cells, at a concentration of 50-200 μg/ml in 5% serum supplemented media.
- Product Form
- Purified Protein - lyophilised
- Use 5-10 ml of sterile physiological saline. Mix gently and transfer to a sterile, screw cap culture tube. Dilute to a final concentration of 50-300 µg/ml in the appropriate serum supplemented media. Solution may be filtered on low protein-binding membranes if necessary.
- 0.1 micron filtered.
- Purified endothelial mitogen from bovine hypothalamus.
- Buffer Solution
- Phosphate buffered saline, pH 7.4
- Preservative Stabilisers
- See Buffer solution.
- Microorganisms - not detected.Mycoplasma - not detected.
- Lyophilised product is stable for 6 months at 4ºC.
Solutions are stable for 2 weeks at 4ºC or 1 month at -20ºC. Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
- Shelf Life
- 6 months from date of despatch.
- For research purposes only
Applications of ECGS
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
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Product Specific References
References for ECGS
Baker, M. et al. (2013) Stromal Claudin14-Heterozygosity, but Not Deletion, Increases Tumour Blood Leakage without Affecting Tumour Growth.
PLoS One. 8: e62516.
Browne, A. et al. (2015) Pharmacological Inhibition of β3 Integrin Reduces the Inflammatory Toxicities Caused by Oncolytic Adenovirus without Compromising Anticancer Activity.
Cancer Res. 75 (14): 2811-21.
Lemaire, M. et al. (2015) Arsenic Exposure Increases Monocyte Adhesion to the Vascular Endothelium, a Pro-Atherogenic Mechanism.
PLoS One. 10 (9): e0136592.
Dieterich, L.C. et al. (2017) Tumor-Associated Lymphatic Vessels Upregulate PDL1 to Inhibit T-Cell Activation.
Front Immunol. 8: 66.
Gittens, B.R. et al. (2017) Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation.
J Immunol. 198 (11): 4458-69.
Pintus, C. et al. (1983) Endothelial cell growth supplement: a cell cloning factor that promotes the growth of monoclonal antibody producing hybridoma cells.
J Immunol Methods. 61 (2): 195-200.