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TNF Alpha

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Dog TNF alpha ELISA

Recombinant Dog TNF alpha (PDP018) detected using Goat anti Dog TNF alpha (AHP2975 ) as the capture reagent and Goat anti Dog TNF alpha:Biotin (AHP2975B ) as the detection reagent followed by Streptavidin:HRP

Recombinant Dog TNF Alpha

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PDP018
Datasheet
E WB
MSDS
5 µg loader

Recombinant Dog tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), is a recombinant protein corresponding to a 157 amino acid sequence of native canine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified.

Canine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the canine inflammatory response.

This product may be used as a standard in immunoassays.

Product Details

Target Species
Dog
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
16.9 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Guarantee
12 months from date of despatch

More Information

UniProt
P51742
Entrez Gene
TNF
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of TNF Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2975 as a coating antibody and AHP2975B as a detection antibody.
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