TNF Alpha
Recombinant Dog TNF alpha (PDP018) detected using Goat anti Dog TNF alpha (AHP2975 ) as the capture reagent and Goat anti Dog TNF alpha:Biotin (AHP2975B ) as the detection reagent followed by Streptavidin:HRP
Recombinant Dog tumor necrosis factor alpha (PDP018) used as a positive control for the stimulation of canine aortic endothelial cells in vitro.
Image caption:
Adhesion molecule protein expression in A. vasorum antigen (AvAg)-stimulated canine endothelial cells. CAEC cells were stimulated with A. vasorum L3 antigen (AvAg; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation, total protein was extracted. Non-stimulated CAEC served as negative controls. The expression of E-selectin (B), P-selectin (C) and VCAM-1 (D) was studied by Western blotting (A); representative illustration of one CAEC isolate and densitometric analysis of protein bands. TNF-α was used as positive control of CAEC stimulation. The detection of vinculin was used as loading control for sample normalization.
From: Grob D, Conejeros I, López-Osorio S, Velásquez ZD, Segeritz L, Gärtner U, Schaper R, Hermosilla C, Taubert A.
Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
Biology (Basel). 2021 May 12;10(5):427.
doi: 10.3390/biology10050427.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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- Product Type
- Recombinant Protein
- Specificity
- TNF Alpha
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Antibody Quality and 
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| Recombinant Dog tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), is a recombinant protein corresponding to a 157 amino acid sequence of native canine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified. Canine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the canine inflammatory response. This product may be used as a standard in immunoassays. |
- Target Species
- Dog
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 16.9 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for TNF Alpha
-
Grob, D. et al. (2021) Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro..
Biology (Basel). 10 (5)May 12 [Epub ahead of print].
- Synonyms
- TNFSF2
- UniProt
- P51742
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
View more products with TNF ALPHA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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