TNF Alpha
Recombinant Dog TNF alpha (PDP018) detected using Goat anti Dog TNF alpha (AHP2975 ) as the capture reagent and Goat anti Dog TNF alpha:Biotin (AHP2975B ) as the detection reagent followed by Streptavidin:HRP
Recombinant Dog tumor necrosis factor alpha (PDP018) used as a positive control for the stimulation of canine aortic endothelial cells in vitro.
Image caption:
Adhesion molecule protein expression in A. vasorum antigen (AvAg)-stimulated canine endothelial cells. CAEC cells were stimulated with A. vasorum L3 antigen (AvAg; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation, total protein was extracted. Non-stimulated CAEC served as negative controls. The expression of E-selectin (B), P-selectin (C) and VCAM-1 (D) was studied by Western blotting (A); representative illustration of one CAEC isolate and densitometric analysis of protein bands. TNF-α was used as positive control of CAEC stimulation. The detection of vinculin was used as loading control for sample normalization.
From: Grob D, Conejeros I, López-Osorio S, Velásquez ZD, Segeritz L, Gärtner U, Schaper R, Hermosilla C, Taubert A.
Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
Biology (Basel). 2021 May 12;10(5):427.
doi: 10.3390/biology10050427.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Dog tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), is a recombinant protein corresponding to a 157 amino acid sequence of native canine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified.
Canine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the canine inflammatory response.
This product may be used as a standard in immunoassays.
Canine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the canine inflammatory response.
This product may be used as a standard in immunoassays.
Product Details
- Target Species
- Dog
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 16.9 kDa
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P51742
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- Regulatory
- For research purposes only
Applications of TNF Alpha
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for TNF Alpha
-
Grob, D. et al. (2021) Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro..
Biology (Basel). 10 (5)May 12 [Epub ahead of print].
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
