MIP-1 Alpha

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Recombinant Bovine MIP-1 Alpha

Product Type: Recombinant Protein
Specificity: MIP-1 Alpha

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Recombinant Bovine MIP-1 alpha , also known as CCL3, is a recombinant protein corresponding to the 24 -93 amino acid sequence of native bovine MIP-1 alpha.

MIP-1 alpha, belonging to the CC chemokine family, plays a critical role in hematopoiesis and is shown to signal through the chemokine receptors CCR1, CCR4 and CCR5.

MIP-1 alpha is also involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as macrophages, NK cells, granulocytes, dendritic cells and T-cells.

Target Species: Bovine
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source: Pichia pastoris
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10% Trehalose
Purity: >95% by SDS PAGE analysis
Protein Molecular Weight: 7.8 kDa
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2996 as a coating antibody and AHP2996B as a detection antibody

Synonyms: CCL3
UniProt: Q8SQA6
Entrez Gene: CCL3
GO Terms: GO:0005615 extracellular space; GO:0006935 chemotaxis; GO:0006954 inflammatory response; GO:0006955 immune response