Recombinant Bovine interleukin-4 may be used as a standard in ELISA assays to determine bovine IL - 4 levels. This product demonstrates functional activity in activation assays utilising bovine B lymphocytes, although a formal ED50 value has not been determined.
- Target Species
- Product Form
- Tissue Culture Supernatant from COS-7 cells expressing recombinant bovine Interleukin - 4 - lyophilised
- Reconstitute with 1 ml distilled water
- Preservative Stabilisers
- 1% Bovine Serum Albumin
The addition of 0.09% w/v sodium azide is recommended for long term storage at 4oC.
- Approx. Protein Concentrations
- rbov IL - 4 at 36 ng/ml
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
interleukin-4 receptor binding
growth factor activity
positive regulation of B cell proliferation
B cell activation
negative regulation of apoptosis
positive regulation of MHC class II biosynthetic process
negative regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate positive/negative controls.
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