IL-4

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Recombinant Bovine Interleukin-4

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP010 E 1 ml
Recombinant Bovine interleukin-4 may be used as a standard in ELISA assays to determine bovine IL - 4 levels. This product demonstrates functional activity in activation assays utilising bovine B lymphocytes, although a formal ED50 value has not been determined.

Product Details

Target Species
Bovine
Product Form
Tissue Culture Supernatant from COS-7 cells expressing recombinant bovine Interleukin - 4 - lyophilised
Reconstitution
Reconstitute with 1 ml distilled water
Preservative Stabilisers
1% Bovine Serum Albumin

The addition of 0.09% w/v sodium azide is recommended for long term storage at 4oC.
Approx. Protein Concentrations
rbov IL - 4 at 36 ng/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P30367 Related reagents
Entrez Gene
IL4 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0030890 positive regulation of B cell proliferation
GO:0042113 B cell activation
GO:0043066 negative regulation of apoptosis
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
Regulatory
For research purposes only

Applications of IL-4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate positive/negative controls.