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IL-13

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Bovine IL-13 ELISA

Recombinant bovine IL-13 (PBP018) detected using Rabbit anti Bovine IL-13 (AHP2374) as the capture reagent and Rabbit anti Bovine IL-13:Biotin (AHP2374B) as the detection reagent followed by Streptavidin:HRP.

Recombinant Bovine Interleukin-13

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PBP018
Datasheet
E
MSDS
5 µg loader

Recombinant Bovine interleukin-13 (IL-13) is a recombinant protein corresponding to aa 21 - 132 of native bovine IL-13.

Bovine interleukin-13 is an immunoregulatory cytokine produced by activated T lymphocytes. IL-13 has a range of activities that include the stimulation of B cell proliferation and immunoglobulin production, as well as the induction of differentiation in both B cells and cells of the monocytic lineage.

Product Details

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
12.6 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9XSV9 Related reagents
Entrez Gene
IL13 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005144 interleukin-13 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IL-13

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2374 as a coating antibody and AHP2374B as a detection antibody.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
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