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IgG1

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ELISA detection of immobilized, purified bovine IgG1 (PBP003) detected with Mouse anti Bovine IgG1 (MCA627GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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Purified Bovine IgG1

Product Type
Purified Protein
Specificity
IgG1

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PBP003
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Purified Bovine IgG1 is a preparation of purified polyclonal bovine IgG1 prepared from normal bovine serum.
By immunoelectrophoresis, Purified Bovine IgG1 (PBP003) reacts with bovine IgG1. No reactivity was observed with antiserum specific for bovine IgG2, IgA or IgM. Purified Bovine IgG1 (PBP003) contains <1% IgG2, IgA and IgM as determined by ELISA.

Target Species
Bovine
Product Form
Purified IgG1 - liquid
Preparation
Purified IgG1 prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG1

  1. Abbott, J. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
    J. Immunol. 174: 6702-6715.

RRID
AB_324783
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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