Gelsolin

Product Details
- Target Species
- Bovine
- Product Form
- Purified protein from bovine plasma - lyophilised
- Reconstitution
- Use sterile distilled water to give a concentrated stock solution => 1 mg/ml.
- Buffer Solution
- Tris buffered saline, EGTA
- Preservative Stabilisers
- See Buffer solution.
- Activity
- 54 U/mg protein.One unit will reduce the viscosity difference between an actin solution and buffer by 50% in a 1 ml reaction mixture containing 1-2 mg F-actin, 0.15 M KCl, 20 mM TRIS, pH 7.6, 0.2 mM CaCl2, 0.2 mM ATP and 1 mM DTT at 28ºC.
- Purity
- SDS PAGE: >95%
- Approx. Protein Concentrations
- 5%, remainder buffer salts.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q3SX14
- Entrez Gene
- GSN
- GO Terms
- GO:0003779 actin binding
- GO:0005856 cytoskeleton
- GO:0051693 actin filament capping
- Regulatory
- For research purposes only
Applications of Gelsolin
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Functional Assays |
Product Specific References
References for Gelsolin
-
Yin, H. L. & Stossel, T. P. (1980) Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.
J. Biol. Chem. 255: 9490-9493. -
Yin, H. L. et al. (1988) Identification of a polyphosphoinositide-modulated domain in gelsolin which binds to the sides of actin filaments.
J. Cell Biol. 106(3): 805-812. -
Sun, H. Q., et al. (1994) The actin side-binding domain of gelsolin also caps actin filaments. Implications for actin filament severing.
J. Biol. Chem. 269(13): 9473-9479. -
Burtnick, L. D., et al. (1997) The crystal structure of plasma gelsolin: implications for actin severing, capping, and nucleation.
Cell 90(4): 661-670.
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