Gelsolin

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Native Bovine Gelsolin

Product Type: Purified Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PBP013 Datasheet	FN SDS	20 µg	Log in		Get Quote

Native Bovine gelsolin is a calcium and polyphosphoinositide regulated actin-binding protein which consists of 6 homologous domains. It is involved in cell morphology, motility, growth and apoptosis. Gelsolin affects filament assembly by severing actin filaments, forming a nucleus for polymerisation, and capping the fast exchanging end of the filaments.

Product Details

Target Species: Bovine
Product Form: Purified protein from bovine plasma - lyophilised
Reconstitution: Use sterile distilled water to give a concentrated stock solution => 1 mg/ml.
Buffer Solution: Tris buffered saline, EGTA
Preservative Stabilisers: See Buffer solution.
Activity: 54 U/mg protein.
One unit will reduce the viscosity difference between an actin solution and buffer by 50% in a 1 ml reaction mixture containing 1-2 mg F-actin, 0.15 M KCl, 20 mM TRIS, pH 7.6, 0.2 mM CaCl2, 0.2 mM ATP and 1 mM DTT at 28ºC.
Purity: SDS PAGE: >95%
Approx. Protein Concentrations: 5%, remainder buffer salts.

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q3SX14
Entrez Gene: GSN
GO Terms: GO:0003779 actin binding; GO:0005856 cytoskeleton; GO:0051693 actin filament capping
Regulatory: For research purposes only

Applications of Gelsolin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Gelsolin

Yin, H. L. & Stossel, T. P. (1980) Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.
J. Biol. Chem. 255: 9490-9493.
Yin, H. L. et al. (1988) Identification of a polyphosphoinositide-modulated domain in gelsolin which binds to the sides of actin filaments.
J. Cell Biol. 106(3): 805-812.
Sun, H. Q., et al. (1994) The actin side-binding domain of gelsolin also caps actin filaments. Implications for actin filament severing.
J. Biol. Chem. 269(13): 9473-9479.
Burtnick, L. D., et al. (1997) The crystal structure of plasma gelsolin: implications for actin severing, capping, and nucleation.
Cell 90(4): 661-670.

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