Gelsolin

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Native Bovine Gelsolin

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP013 FN 20 µg
Native Bovine gelsolin is a calcium and polyphosphoinositide regulated actin-binding protein which consists of 6 homologous domains. It is involved in cell morphology, motility, growth and apoptosis. Gelsolin affects filament assembly by severing actin filaments, forming a nucleus for polymerisation, and capping the fast exchanging end of the filaments.

Product Details

Target Species
Bovine
Product Form
Purified protein from bovine plasma - lyophilised
Reconstitution
Use sterile distilled water to give a concentrated stock solution => 1 mg/ml.
Buffer Solution
Tris buffered saline, EGTA
Preservative Stabilisers
See Buffer solution.
Activity
54 U/mg protein.
One unit will reduce the viscosity difference between an actin solution and buffer by 50% in a 1 ml reaction mixture containing 1-2 mg F-actin, 0.15 M KCl, 20 mM TRIS, pH 7.6, 0.2 mM CaCl2, 0.2 mM ATP and 1 mM DTT at 28ÂșC.
Purity
SDS PAGE: >95%
Approx. Protein Concentrations
5%, remainder buffer salts.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of despatch.

More Information

UniProt
Q3SX14 Related reagents
Entrez Gene
GSN Related reagents
GO Terms
GO:0003779 actin binding
GO:0005856 cytoskeleton
GO:0051693 actin filament capping
Regulatory
For research purposes only

Applications of Gelsolin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Gelsolin

  1. Yin, H. L. & Stossel, T. P. (1980) Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.
    J. Biol. Chem. 255: 9490-9493.
  2. Yin, H. L. et al. (1988) Identification of a polyphosphoinositide-modulated domain in gelsolin which binds to the sides of actin filaments.
    J. Cell Biol. 106(3): 805-812.
  3. Sun, H. Q., et al. (1994) The actin side-binding domain of gelsolin also caps actin filaments. Implications for actin filament severing.
    J. Biol. Chem. 269(13): 9473-9479.
  4. Burtnick, L. D., et al. (1997) The crystal structure of plasma gelsolin: implications for actin severing, capping, and nucleation.
    Cell 90(4): 661-670.