Borrelia burgdorferi

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Native Borrelia burgdorferi

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1440-0006 E 1 mg
Borrelia burgdorferi is a spirochete, the causitive agent of the tick borne zoonotic disease Borreliosis or Lyme disease. The organism was first isolated in 1982 by Willy Burgdorfer after whom the organism was named. B. burgdorferi is the principal cause of Lyme disease in the U.S while B. afzelii and B. garinii are found as causitive agents in Europe.

Product Details

Target Species
Bacterial
Product Form
Sonicated organism suspension of B. burgdorferi (strain B31) - liquid
Preparation
Prepared from B. burgdorferi strain B31 grown in BSK11 medium. The preparation contains all major antigens with residual cellular debris. Considered inactive when no detection of growth in two blind passages under original culture conditions is observed. Rendered inactive using gamma irradiation.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Approx. Protein Concentrations
0.8 mg/ml using the Biorad dye binding assay. Standard curve is generated with a known concentration of IgG.

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Borrelia burgdorferi

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Instructions For Use
This preparation should be sonicated immediately prior to use to ensure that the preparation is uniform.

Application Based External Images

Immunoblotting

Product Specific References

References for Borrelia burgdorferi

  1. Zeman, D. et al. (2012) Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern.
    Fluids Barriers CNS. 9: 5.