Borrelia burgdorferi is a spirochete, the causitive agent of the tick borne zoonotic disease Borreliosis or Lyme disease. The organism was first isolated in 1982 by Willy Burgdorfer after whom the organism was named. B. burgdorferi is the principal cause of Lyme disease in the U.S while B. afzelii and B. garinii are found as causitive agents in Europe.
- Target Species
- Product Form
- Sonicated organism suspension of B. burgdorferi (strain B31) - liquid
- Prepared from B. burgdorferi strain B31 grown in BSK11 medium. The preparation contains all major antigens with residual cellular debris. Considered inactive when no detection of growth in two blind passages under original culture conditions is observed. Rendered inactive using gamma irradiation.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Approx. Protein Concentrations
- 0.8 mg/ml using the Biorad dye binding assay. Standard curve is generated with a known concentration of IgG.
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
- Shelf Life
- 12 months from date of despatch
- For research purposes only
Applications of Borrelia burgdorferi
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Instructions For Use
- This preparation should be sonicated immediately prior to use to ensure that the preparation is uniform.
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Product Specific References
References for Borrelia burgdorferi
Zeman, D. et al. (2012) Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern.
Fluids Barriers CNS. 9: 5.