GCLC antibody
Rabbit anti GCLC
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- GCLC
Antibody Quality and Performance Guarantee
| Rabbit anti Human GCLC antibody recognizes glutamate-cysteine ligase catalytic subunit, also known as GCS heavy chain, gamma-ECS or gamma-glutamylcysteine synthetase. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction (provided by RefSeq, Oct 2010). Rabbit anti Human GCLC antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Rabbit anti GCLC detects a band of approximately 75 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide encompassing part of the middle region of human GCLC
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
- UniProt
- P48506
- Entrez Gene
- GCLC
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004357 glutamate-cysteine ligase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0045454 cell redox homeostasis
- GO:0006534 cysteine metabolic process
- GO:0006536 glutamate metabolic process
- GO:0006750 glutathione biosynthetic process
- GO:0006805 xenobiotic metabolic process
- View More GO Terms
- GO:0006916 anti-apoptosis
- GO:0006979 response to oxidative stress
- GO:0016595 glutamate binding
- GO:0009408 response to heat
- GO:0009725 response to hormone stimulus
- GO:0016481 negative regulation of transcription
- GO:0043531 ADP binding
- GO:0050662 coenzyme binding
- GO:0050880 regulation of blood vessel size
VPA00695
161208
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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