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Protein Kinase C Epsilon antibody | CD04/1C7

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Anti Protein Kinase C Epsilon Antibody, clone CD04/1C7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Protein Kinase C Epsilon Antibody, clone CD04/1C7 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti Protein Kinase C Epsilon Antibody, clone CD04/1C7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with protein kinase C epsilon antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to protein kinase C epsilon (molecular weight 91 kDa).
Anti Protein Kinase C Epsilon Antibody, clone CD04/1C7 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of Protein Kinase C Epsilon from mouse brain lysate. The Input lane is 10% input; the Protein Kinase C Epsilon-IP lane is the immunoprecipitation with the Protein Kinase C Epsilon antibody (2.5 µg, VMA00636); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA691). Western blot analysis was performed using Protein Kinase C Epsilon antibody (1/1,000, VMA00636) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000,1705047) and visualized on the ChemiDoc MP. Arrow points to Protein Kinase C Epsilon.

Mouse anti Protein Kinase C Epsilon

Product Type
PrecisionAb Monoclonal
Clone
CD04/1C7
Isotype
IgG2b
Format
Purified
Specificity
Protein Kinase C Epsilon

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Product Code Applications Pack Size List Price Your Price Qty
VMA00636
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human protein kinase C epsilon antibody recognizes protein kinase C epsilon type also known PKCE.

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by PRKCE is one of the PKC family members. This kinase is involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior (provided by RefSeq, Jul 2008).

Mouse anti Human protein kinase C epsilon antibody detects a band of 91 kDa. The antibody has been extensively validated for western blotting using whole cell and tissue lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti protein kinase C epsilon antibody detects a band of approximately 91 kDa in mouse brain lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human protein kinase C epsilon (aa 301-737)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q02156
Entrez Gene
PRKCE
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0006917 induction of apoptosis
GO:0007202 activation of phospholipase C activity
GO:0008047 enzyme activator activity
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0048011 nerve growth factor receptor signaling pathway

VMA00636

170517

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