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Heat Shock 70 Kda Protein 5 antibody | CD01/1A7

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Anti Heat Shock 70 Kda Protein 5 Antibody, clone CD01/1A7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Heat Shock 70 Kda Protein 5 Antibody, clone CD01/1A7 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti Heat Shock 70 Kda Protein 5 Antibody, clone CD01/1A7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with heat shock 70 kDa protein 5 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to heat shock 70 kDa protein 5 (molecular weight 78 kDa)
Anti Heat Shock 70 Kda Protein 5 Antibody, clone CD01/1A7 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of heat shock 70 kDa protein 5 from HeLa whole cell lysate. The Input lane is 10% input; the HEAT SHOCK 70 kDa PROTEIN 5-IP lane is the immunoprecipitation with the heat shock 70 kDa protein 5 antibody (2.5 µg, VMA00570); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using heat shock 70 kDa protein 5 antibody (1/1,000, VMA00570) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to heat shock 70 kDa protein 5.

Mouse anti Heat Shock 70 Kda Protein 5

Product Type
PrecisionAb Monoclonal
Clone
CD01/1A7
Isotype
IgG1
Format
Purified
Specificity
Heat Shock 70 Kda Protein 5

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Product Code Applications Pack Size List Price Your Price Qty
VMA00570
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human heat shock 70 kDa protein 5 antibody recognizes heat shock 70 kDa protein 5 also known as GRP78, HSPA5 or 78 kDa glucose-regulated protein.

Heat shock 70 kDa protein 5 is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

Mouse anti Human heat shock 70 kDa Protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human heat shock 70 kDa protein 5 (amino acids 1 - 654)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P11021
Entrez Gene
HSPA5
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0030674 protein binding, bridging
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0006916 anti-apoptosis
GO:0009986 cell surface
GO:0030168 platelet activation
GO:0030176 integral to endoplasmic reticulum membrane
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031625 ubiquitin protein ligase binding
GO:0042149 cellular response to glucose starvation
GO:0042470 melanosome
GO:0043027 caspase inhibitor activity
GO:0043154 negative regulation of caspase activity
GO:0048471 perinuclear region of cytoplasm
GO:0060904 regulation of protein folding in endoplasmic reticulum

VMA00570

170420

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