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MCM3 antibody | 1593CT377.41.73

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Anti MCM3 Antibody, clone 1593CT377.41.73 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti MCM3 Antibody, clone 1593CT377.41.73 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti MCM3 Antibody, clone 1593CT377.41.73 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with MCM3 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 12 second exposure. Arrow points to MCM3 (molecular weight 105 kDa).
Anti MCM3 Antibody, clone 1593CT377.41.73 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of MCM3 from Jurkat whole cell lysate. The Input lane is 10% input; the MCM3-IP lane is the immunoprecipitation with the MCM3 antibody (2.5 µg, VMA00487); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 μg, MCA928). Western blot analysis was performed using MCM3 antibody (1/1,000, VMA00487) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to MCM3.

Mouse anti MCM3

Product Type
PrecisionAb Monoclonal
Clone
1593CT377.41.73
Isotype
IgG1
Format
Purified
Specificity
MCM3

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Product Code Applications Pack Size List Price Your Price Qty
VMA00487
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human MCM3 antibody recognizes the DNA replication licensing factor MCM3, also known as DNA polymerase alpha holoenzyme-associated protein P1, MCM3 minichromosome maintenance deficient 3, cervical cancer proto-oncogene 5 or replication licensing factor, beta subunit.

The protein encoded by MCM3 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for MCM3 (provided by RefSeq, Jul 2012).

Mouse anti Human MCM3 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MCM3 detects a band of approximately 105 kDa in Jurkat cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein of human MCM3
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P25205
Entrez Gene
MCM3
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0003677 DNA binding
GO:0017111 nucleoside-triphosphatase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005658 alpha DNA polymerase:primase complex
GO:0005813 centrosome
GO:0006270 DNA-dependent DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006350 transcription
GO:0045449 regulation of transcription
GO:0048471 perinuclear region of cytoplasm

VMA00487

160712

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