Cytokeratin 18 antibody | AbD25791
Human anti Cytokeratin 18
- Product Type
- PrecisionAb Monoclonal
- Clone
- AbD25791
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
- Specificity
- Cytokeratin 18
Antibody Quality and Performance Guarantee
| Human anti Human cytokeratin 18 antibody recognizes cytokeratin 18, also known as K18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, keratin type I cytoskeletal 18 or simply keratin-18. Encoded by the KRT18 gene, cytokeratin 18 together with its filament partner cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in KRT18 have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for KRT18 (provided by RefSeq, Jul 2008). Human anti Human cytokeratin 18 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti cytokeratin 18 detects a band of approximately 46 kDa in MCF7 cell lysates.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase (BAP) fusion protein. The BAP enzymatic activity is silenced by mutation. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts.
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- Cytokeratin 18
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
- UniProt
- P05783
- Entrez Gene
- KRT18
- GO Terms
- GO:0005515 protein binding
- GO:0005198 structural molecule activity
- GO:0007049 cell cycle
- GO:0009653 anatomical structure morphogenesis
- GO:0034451 centriolar satellite
- GO:0043000 Golgi to plasma membrane CFTR protein transport
- GO:0043066 negative regulation of apoptosis
- GO:0044419 interspecies interaction between organisms
- GO:0045095 keratin filament
- View More GO Terms
- GO:0048471 perinuclear region of cytoplasm
VMA00444
160226
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CYTOKERATIN specificity
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