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PSMC5 antibody | J02/1D1

Mouse anti PSMC5

Product Type
PrecisionAb Monoclonal
Clone
J02/1D1
Isotype
IgG1
Format
Purified
Specificity
PSMC5

Product Code Applications Pack Size List Price Your Price Qty
VMA00329
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human PSMC5 antibody recognizes the 26S protease regulatory subunit 8, also known as 26S protease regulatory subunit 8, 26S proteasome AAA-ATPase subunit RPT6, MSUG1 protein, Tat-binding protein homolog 10, proteasome 26S ATPase subunit 5, proteasome 26S subunit ATPase 5, proteasome subunit p45, thyroid hormone receptor-interacting protein 1, thyroid receptor interactor 1.

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMC5 gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for PSMC5 (provided by RefSeq, Nov 2010).

Mouse anti Human PSMC5 detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PSMC5 detects a band of approximately 46 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein corresponding to amino acids 1-130 of human PSMC5 expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P62195
Entrez Gene
PSMC5
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000502 proteasome complex
GO:0003712 transcription cofactor activity
GO:0006915 apoptosis
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006366 transcription from RNA polymerase II promoter
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0008134 transcription factor binding
GO:0016887 ATPase activity
GO:0016032 viral reproduction
GO:0016071 mRNA metabolic process
GO:0042981 regulation of apoptosis
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0031531 thyrotropin-releasing hormone receptor binding
GO:0043069 negative regulation of programmed cell death
GO:0043193 positive regulation of gene-specific transcription
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle

VMA00329

160115

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