EBP50 antibody | EBP-10
KO Validated
Mouse anti EBP50
- Product Type
- PrecisionAb Monoclonal
- Clone
- EBP-10
- Isotype
- IgG2b
- Format
- Purified
- Specificity
- EBP50
Antibody Quality and Performance Guarantee
| Mouse anti Human EPB50 antibody recognizes ezrin/radixin/moesin (ERM)-binding phosphoprotein of 50 kDa (EBP50) otherwise known as Na+/H+ exchanger regulatory factor 1 (NHERF1), regulatory cofactor of Na(+)/H(+) exchanger, sodium-hydrogen exchanger regulatory factor 1 or solute carrier family 9 isoform A3 regulatory factor 1. EBP50 is a 358 amino acid cytoplasmic adaptor molecule containing two PDZ domains. EBP50 is expressed in tissues containing polarized epithelia, including the small intestine, placenta, liver and kidney. The ERM family of membrane-cytoskeletal linking proteins act to maintain a regulated linkage between actin filaments (F-actin) and the plasma membrane. EBP50 is an important ERM binding partner, which interacts with the N-terminal FERM (four point one ERM) domain of ERM proteins, via its C-terminal domain. EBP50 colocalizes with actin and ezrin, in the apical mircovilli of epithelial cells and this role as binding partner has been further shown through the interaction of EBP50 with the transmembrane adaptor protein known as protein associated with glycosphingolipid-enriched microdomains (PAG), in T-cells (Reczek et al. 1997). Mutations in the gene encoding EBP50 leads to nephrolithiasis/osteoporosis, hypophosphatemic, 2 disease, characterized by impaired phosphate metabolism (Courbebaisse et al. 2012). Other ligands which bind to the PDZ domains of EBP50, include beta-catenin, cystic fibrosis transmembrane conductance regulator and the type II Na/Pi cotransporter Npt2, implicating EBP50 in a diverse range of biological systems. Mouse anti Human EBP50 antibody recognizes EBP50 as a single band of ~50 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney. |
|
- Target Species
- Human
- Western Blotting
- Anti EBP50 antibody detects a band of approximately 50 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- Bacterially produced recombinant full length human EBP50.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- O14745
- Entrez Gene
- SLC9A3R1
- GO Terms
- GO:0001726 ruffle
- GO:0016055 Wnt receptor signaling pathway
- GO:0005813 centrosome
- GO:0005902 microvillus
- GO:0006461 protein complex assembly
- GO:0012505 endomembrane system
- GO:0015629 actin cytoskeleton
- GO:0016324 apical plasma membrane
- GO:0030165 PDZ domain binding
- View More GO Terms
- GO:0030175 filopodium
- GO:0031698 beta-2 adrenergic receptor binding
- GO:0032415 regulation of sodium:hydrogen antiporter activity
- GO:0043231 intracellular membrane-bounded organelle
VMA00054
230914
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