Durvalumab antibody | AbD41240
Human anti Durvalumab (Drug/Target Complex):HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD41240
- Isotype
- Fab antibody
- Specificity
- Durvalumab
- Region
- DRUG/TARGET COMPLEX
Human Anti-Durvalumab (Drug/Target Complex) Antibody, clone AbD41240 specifically recognizes the antibody drug durvalumab when in complex with its target, PD-L1 (programmed cell death ligand 1). The antibody does not recognize free durvalumab nor unbound recombinant human PD-L1. It can be used to measure levels of durvalumab in patient samples. This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA002) and bivalent Fab conjugated to HRP (TZA002P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free durvalumab captured via immobilized PD-L1. The antibody is tagged with a SpyTag2 at the C-terminus of the Fab antibody heavy chain, enabling the user to couple it to any of the SpyCatchers in our catalog for conversion to alternative formats in less than one hour. View a summary of all SpyCatcher products Durvalumab (Imfinzi) is a human IgG1/kappa antibody with allotype G1m3. The CH2 domain was engineered with three amino acid substitutions (L238F, L239E, P335S) to reduce the Fc-mediated effector functions to prevent depletion of PD-L1+ T-cells. It was first approved for previously treated patients with advanced bladder cancer. It was later approved for the treatment of unresectable stage 3 NSCLC (Non-Small Cell Lung Cancer) and ES-SCLC. Durvalumab binds to PD-L1 to block the interaction with the inhibitory T cell receptor PD-1. This blockade removes the suppression of T-cells that are specific for MHC-1-presented neoepitopes on tumor cells. Removal of this inhibitory signal leads to the release of granzymes and perforins by the activated T-cells, and finally to the destruction of the tumor cells. View a summary of all anti-durvalumab antibodies |
- Product Form
- A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA002 with a HRP conjugated BiSpyCatcher version 2. The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- PD-L1/Durvalumab complex
- Affinity
- The monovalent intrinsic affinity of AbD41240pap was measured as KD = 13 nM by real time, label free molecular interaction analysis on immobilized durvalumab in complex with PD-L1.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Imfinzi is a trademark of Medimmune/AstraZeneca using Abgenix`s XenoMouse® technology
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD41240pap can be used in a pharmacokinetic (PK) antigen capture ELISA to measure durvalumab captured via immobilized human PD-L1 or to measure the level of durvalumab/PD-L1 complex.
Protocol: PK Antigen Capture ELISA.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
SpyTag3 Peptide | BLP086 | IY | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | SpyTag3 Peptide | ||||||
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Hispec Assay Diluent | ||||||
Human anti Durvalumab | HCA359 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Durvalumab | ||||||
Human anti Durvalumab | HCA360 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Durvalumab | ||||||
Human anti Durvalumab (Drug/Target Complex) | TZA002 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Durvalumab (Drug/Target Complex) | ||||||
Human anti Durvalumab | TZA004 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Durvalumab |
- Synonyms
- Imfinzi
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
TZA002P
163203 166334If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up