CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9
Triple color anti Canine CD3/CD4/CD8 antibody cocktail (TC014) used for gating lymphocyte populations from canine blood cell cultures by flow cytometry.
Image caption:
Representative dot plots demonstrating gating strategy of lymphocytes expressing (isotype controls and test samples) CD3 and CD4 (A), CD4 and CD8 (B), CD21 (C), CD4 and CD25 (D), CD25 and FoxP3 (E) after a 72 h culture of PBMC in a 37°C, 5% CO2 environment and PHA (mitogen).
From:Guzera M, Szulc-Dąbrowska L, Cywińska A, Archer J, Winnicka A (2016)
In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
PLoS ONE 11(5): e0154429.
doi: 10.1371/journal.pone.0154429
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647
- Product Type
- Triple Color Reagent
- Clone
- CA17.2A12 / YKIX302.9 / YCATE55.9
- Isotype
- Cocktail
- Specificity
- CD3/CD4/CD8
Antibody Quality and Performance Guarantee
Anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647 is a triple color reagent recognizing canine CD3, CD4 and CD8 cell surface antigens. FITC conjugated Mouse anti Canine CD3 antibody, clone CA17.2A12 (MCA1477F) recognizes canine CD3, expressed by T lymphocytes. RPE conjugated Mouse anti Canine CD4 antibody, clone YKIX 302.9 (MCA1038PE) recognizes canine CD4, expressed by lymphocyte subsets and canine neutrophils. Alexa Fluor®647 conjugated Mouse anti Canine CD8 antibody, clone YCATE55.9 (MCA1039A647) recognizes CD8 alpha, expressed by cytotoxic T cells. |
- Target Species
- Dog
- Product Form
- Triple Color combination consisting of FITC, RPE, Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- CD3 FITC reagent: Mouse IgG1CD4 RPE reagent: Rat IgG2aCD8 Alexa Fluor® 647 reagent: Rat IgG1
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:Alexa Fluor® 647 -ve Control | TC023 | F * | 100 Tests/1ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:Alexa Fluor® 647 -ve Control | ||||||
References for CD3/CD4/CD8
-
Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
J Vet Med Sci. 68:1219-1221 -
Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
PLoS One. 11 (5): e0154429. -
Maina, E. et al. (2019) Food allergen-specific sublingual immunotherapy modulates peripheral T cell responses of dogs with adverse food reactions.
Vet Immunol Immunopathol. 212: 38-42. -
Franco-Molina, M.A. et al. (2018) Autologous canine immunotherapy: short-time generated dendritic cells loaded with canine transmissible venereal tumor-whole lysate.
Immunopharmacol Immunotoxicol. 40 (5): 437-43. -
Jaensch, S.M. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia.
Aust Vet J. 100 (11): 527-32.
- RRID
- AB_808411
- UniProt
- P27597
- P33705
- P33706
- Entrez Gene
- CD3E
- CD4
- CD8A
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007155 cell adhesion
- GO:0006955 immune response
- GO:0045058 T cell selection
TC014
149664
156121
161311
163375
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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